@article{uoadl:2981631,
    volume = "34",
    number = "18",
    pages = "2528-2537",
    journal = "Journal of High Resolution Chromatography (now called Journal of Separation Science)",
    issn = "0935-6304, 1521-4168",
    keywords = "Biphasic solvent system;  Countercurrent chromatography;  Crude rice bran oil;  Dual modes;  Ferulates;  Large-scale isolation;  LTQ-Orbitrap;  Non-aqueous;  One step;  Oryzanol;  Rice bran oil;  Target compound;  Triterpenes, Acetonitrile;  Hydraulics;  Hydrodynamics;  Hydroxylation;  Liquid chromatography;  Organic solvents, Furfural, acetonitrile;  butanol;  gamma oryzanol;  heptane;  triterpene, article;  chemical analysis;  counter current chromatography;  fractionation;  high performance liquid chromatography;  hydroxylation;  nonhuman;  priority journal;  quantitative analysis;  rice bran, Countercurrent Distribution;  Molecular Structure;  Phenylpropionates;  Plant Oils;  Stereoisomerism",
    BIBTEX_ENTRY = "article",
    year = "2011",
    author = "Angelis, A. and Urbain, A. and Halabalaki, M. and Aligiannis, N. and Skaltsounis, A.-L.",
    abstract = "The value-added γ-oryzanol was purified in one step from crude rice bran oil (RBO) using a preparative hydrostatic countercurrent chromatography (hydrostatic CCC) method, operating in the dual mode. The fractionation was performed using a non-aqueous biphasic solvent system consisting of heptane-acetonitrile-butanol (1.8:1.4:0.7, v/v/v), leading rapidly to the target compounds. Transfer of the analytical CCC method to large-scale isolation was also carried out yielding a high quantity-high purity fraction of γ-oryzanol. In addition, a fraction of hydroxylated triterpene alcohol ferulates (polar γ-oryzanol) was clearly separated and obtained. Furthermore, a fast HPLC-APCI(±)-HRMS method was developed and applied for the identification of γ-oryzanol as well as the polar γ-oryzanol in RBO and the resulting fractions. The purity of γ-oryzanol fraction was estimated as 97% based on HPLC-APCI-HRMS analysis. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.",
    title = "One-step isolation of γ-oryzanol from rice bran oil by non-aqueous hydrostatic countercurrent chromatography",
    doi = "10.1002/JSSC.201100192"
}