@article{uoadl:3023324,
    volume = "21",
    number = "12",
    pages = "1783-1795",
    journal = "JOURNAL OF LIQUID CHROMATOGRAPHY AND RELATED TECHNOLOGIES",
    issn = "1082-6076",
    keywords = "Acetic acid;  Degradation;  Drug products;  Hydrochloric acid;  Hydrolysis;  Mass spectrometry;  Nuclear magnetic resonance spectroscopy;  pH;  Solutions;  Ultraviolet detectors, Acidic hydrolysis;  Chlorochlorophenyl quinazolinecarboxaldehyde;  Degradation product;  Lorazepam, High performance liquid chromatography, lorazepam, article;  carbon nuclear magnetic resonance;  drug degradation;  drug determination;  high performance liquid chromatography;  hydrolysis;  proton nuclear magnetic resonance;  structure analysis",
    BIBTEX_ENTRY = "article",
    year = "1998",
    author = "Panderi, I.E. and Archontaki, H.A. and Gikas, E.E. and Parissi-Poulou, M.",
    abstract = "A high performance liquid chromatographic method was developed for the kinetic investigation on the acidic hydrolysis of lorazepam in 0.01, 0.1, and 1.0 M hydrochloric acid solutions. In this study, the simultaneous determination of lorazepam and its main degradation product was performed on a reversed phase BDS C-8 column. The mobile phase consisted of a mixture of methanol acetonitrile buffer solution containing 0.005 M KH2PO4 and 0.1 M CH3COONH4 adjusted to pH 6.0 with glacial acetic acid (35 : 20 : 45, v/v/v) pumped at a flow rate of 1.5 mL/min. The UV detector was operated at 230 nm. The main degradation product attained under the above mentioned experimental conditions was 6-chloro-4-(2-chlorophenyl)-2-quinazolinecarboxaldehyde which was isolated and identified by 1H-, 13C-NMR and mass spectrometry. The proposed method showed a relative standard deviation of less than 2.04% and detection limits of 0.16 and 0.17 μg/mL for lorazepam and its main degradation product, respectively.
A high performance liquid chromatographic method was developed for the kinetic investigation on the acidic hydrolysis of lorazepam in 0.01, 0.1, and 1.0 M hydrochloric acid solutions. In this study, the simultaneous determination of lorazepam and its main degradation product was performed on a reversed phase BDS C-8 column. The mobile phase consisted of a mixture of methanol : acetonitrile : buffer solution containing 0.005 M KH2PO4 and 0.1 M CH3COONH4 adjusted to pH 6.0 with glacial acetic acid (35 : 20 : 45, v/v/v) pumped at a flow rate of 1.5 mL/min. The UV detector was operated at 230 nm. The main degradation product attained under the above mentioned experimental conditions was 6-chloro-4-(2-chlorophenyl)-2-quinazolinecarboxaldehyde which was isolated and identified by 1H-, 13C-NMR and mass spectrometry. The proposed method showed a relative standard deviation of less than 2.04% and detection limits of 0.16 and 0.17 μg/mL for lorazepam and its main degradation product, respectively.",
    title = "Kinetic investigation on the degradation of lorazepam in acidic aqueous solutions by high performance liquid chromatography",
    doi = "10.1080/10826079808005891"
}