@article{uoadl:3068056,
    volume = "1031",
    number = "1-2",
    pages = "319-324",
    journal = "Journal of Chromatography A",
    keywords = "Calibration;  Organic compounds;  Particle size analysis, Ultraviolet (UV) filters, High performance liquid chromatography, 4 methoxycinnamic acid 2 ethylhexyl ester;  acetonitrile;  alkene derivative;  benzotriazole derivative;  excipient;  methanol;  methylene bisbenzotriazolyltetramethylphenol;  octocrylene;  salicylic acid octyl ester;  sunscreen;  unclassified drug, aqueous solution;  article;  calibration;  device;  elution;  filter;  flow rate;  particle size;  priority journal;  reversed phase high performance liquid chromatography;  sampling;  standardization;  time;  ultraviolet radiation;  validation process",
    BIBTEX_ENTRY = "article",
    year = "2004",
    author = "Smyrniotakis, C.G. and Archontaki, H.A.",
    abstract = "A non-aqueous reversed-phase high performance liquid chromatographic method (RP-HPLC) with UV detection at 313nm was developed and validated for simultaneous determination of methylene bis-benzotriazolyl tetramethylphenol (Tinosorb M) along with three other chemical UV filters, octocrylene (Eusolex OCR), octyl methoxycinnamate (Eusolex 2292) and octyl salicylate (Eusolex OS) in suncare products. An isocratic elution was performed on a Hypersil BDS RP-C18 column (250mm x 4.6mm), 5μm particle size, using a mobile phase consisted of methanol-acetonitrile (90:10, v/v) with a flow-rate of 1.5ml/min. The determination of the four UV filters was not interfered by the excipients in the products. The method of external standard, as well as the standard addition method was used for the determination. The external standard calibration curves were linear for Eusolex OCR, Eusolex 2292, Eusolex OS, and Tinosorb M in the concentration ranges of 0.5-100μM, 0.5-100μM, 0.5-200μM, and 0.2-100μM, respectively. Day-to-day relative standard deviation of the determination was within 3%. Limits of detection and quantitation of the above compounds were found equal to 36 and 110nM, 220 and 660nM, 170 and 520nM, 44s and 130nM, respectively. The recovery of these four chemical UV filters from the spiked samples was 96-103%. © 2003 Elsevier B.V. All rights reserved.",
    title = "Development and validation of a non-aqueous reversed-phase high-performance liquid chromatographic method for the determination of four chemical UV filters in suncare formulations",
    doi = "10.1016/J.CHROMA.2003.08.078"
}