@article{2981463,
    title = "A fully validated method for the determination of lacosamide in human plasma using gas chromatography with mass spectrometry: Application for therapeutic drug monitoring",
    author = "Nikolaou, P. and Papoutsis, I. and Spiliopoulou, C. and Voudris, C. and Athanaselis, S.",
    journal = "Journal of High Resolution Chromatography (now called Journal of Separation Science)",
    year = "2015",
    volume = "38",
    number = "2",
    pages = "260-266",
    publisher = "Wiley-VCH Verlag",
    issn = "0935-6304, 1521-4168",
    doi = "10.1002/jssc.201400858",
    keywords = "Chromatographic analysis;  Chromatography;  Drug products;  Extraction;  Mass spectrometry;  Patient monitoring;  Patient treatment;  Phase separation;  Plasma (human);  Plasmas;  Spectrometry, Chromatographic methods;  Correlation coefficient;  Epilepsy;  Lacosamide;  Limit of quantifications;  Relative standard deviations;  Solid-phase extraction;  Therapeutic drug monitoring, Gas chromatography, acetonitrile;  chloride;  lacosamide;  n methyl n tert butyldimethylsilytrifluoroacetamide;  tert butyldimethylsilylchloride;  trifluoroacetamide derivative;  unclassified drug;  acetamide derivative;  anticonvulsive agent;  harkoseride, accuracy;  anticonvulsant therapy;  Article;  assay;  drug monitoring;  gas chromatography;  human;  limit of quantitation;  mass spectrometry;  plasma;  priority journal;  solid phase extraction;  blood;  calibration;  drug monitoring;  epilepsy;  mass fragmentography;  procedures;  quality control;  validation study, Acetamides;  Anticonvulsants;  Calibration;  Drug Monitoring;  Epilepsy;  Gas Chromatography-Mass Spectrometry;  Humans;  Quality Control",
    abstract = "A simple gas chromatographic method with mass spectrometry detection was developed and validated for the determination of lacosamide in human plasma. Lacosamide and the internal standard, levetiracetam-d6, were extracted from 200 μL plasma, by a solid-phase extraction through HF Bond Elut C18 columns, and derivatized using N-methyl-N-tert-butyldimethylsilyltrifluoroacetamide with 1% tert-butyldimethylsilylchloride in acetonitrile. The limit of quantification was found to be 0.20 μg/mL and the assay was linear up to 20.0 μg/mL with correlation coefficient ≥0.994. The intra- and interday precision values were <4.1% in terms of relative standard deviation (%) and the values of intra- and interday accuracy were found to be within –7.2 and 5.3% in terms of relative error (%). Absolute recovery of the method for lacosamide was determined at three concentration levels and ranged from 92.5 to 97.6%. The developed method uses small volumes of plasma and proved to be simple, rapid, and sensitive for the determination of lacosamide in plasma. This method can be used in routine every day analysis of plasma samples obtained from patients who follow respective antiepileptic treatment and for the investigation of clinical and forensic cases where lacosamide is involved. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim"
}