@article{2981568,
    title = "Orthogonal patterning of multiple biomolecules using an organic fluorinated resist and imprint lithography",
    author = "Midthun, K.M. and Taylor, P.G. and Newby, C. and Chatzichristidi, M. and Petrou, P.S. and Lee, J.-K. and Kakabakos, S.E. and Baird, B.A. and Ober, C.K.",
    journal = "Biomacromolecules",
    year = "2013",
    volume = "14",
    number = "4",
    pages = "993-1002",
    issn = "1525-7797, 1526-4602",
    doi = "10.1021/bm301783t",
    keywords = "High resolution;  Hydrofluoroether;  Imaging materials;  Imprint lithography;  Lithographic patterning;  Patterning process;  Processing systems;  Room temperature, Nanoimprint lithography, Biomolecules, biomaterial;  DNA;  ether derivative;  monoclonal antibody;  protein;  solvent;  streptavidin, article;  binding affinity;  chemical reaction;  controlled study;  fluorination;  glass transition temperature;  lithography;  molecular imprinting;  molecular stability;  molecule;  priority journal;  process development;  processing;  radiation exposure;  room temperature;  synthesis;  ultraviolet radiation, Animals;  Antibodies, Monoclonal;  Cell Line, Tumor;  DNA;  Hybridization, Genetic;  Leukemia, Basophilic, Acute;  Methacrylates;  Molecular Imprinting;  Nanotechnology;  Proteins;  Rats;  Streptavidin;  Surface Properties",
    abstract = "The ability to spatially deposit multiple biomolecules onto a single surface with high-resolution while retaining biomolecule stability and integrity is critical to the development of micro- and nanoscale biodevices. While conventional lithographic patterning methods are attractive for this application, they typically require the use of UV exposure and/or harsh solvents and imaging materials, which may be damaging to fragile biomolecules. Here, we report the development of a new patterning process based on a fluorinated patterning material that is soluble in hydrofluoroether solvents, which we show to be benign to biomolecules, including proteins and DNA. We demonstrate the implementation of these materials into an orthogonal processing system for patterning multibiomolecule arrays by imprint lithography at room temperature. We further showcase this method's capacity for fabricating patterns of receptor-specific ligands for fundamental cell studies. © 2013 American Chemical Society."
}