@article{2998088,
    title = "Hippocampal lipidome and transcriptome profile alterations triggered by acute exposure of mice to GSM 1800 MHz mobile phone radiation: An exploratory study",
    author = "Fragopoulou, A.F. and Polyzos, A. and Papadopoulou, M.-D. and Sansone, A. and Manta, A.K. and Balafas, E. and Kostomitsopoulos, N. and Skouroliakou, A. and Chatgilialoglu, C. and Georgakilas, A. and Stravopodis, D.J. and Ferreri, C. and Thanos, D. and Margaritis, L.H.",
    journal = "Brain and Behavior",
    year = "2018",
    volume = "8",
    number = "6",
    publisher = "John Wiley and Sons Ltd",
    issn = "2162-3279",
    doi = "10.1002/brb3.1001",
    keywords = "dimethyl disulfide;  docosahexaenoic acid;  docosapentaenoic acid;  fatty acid ester;  icosapentaenoic acid;  linolenic acid;  lipidome;  monounsaturated fatty acid;  palmitic acid;  palmitoleic acid;  saturated fatty acid;  transcriptome;  ethyl eicosapentaenoic acid;  fatty acid;  icosapentaenoic acid;  monounsaturated fatty acid;  transcriptome, adult;  animal behavior;  animal experiment;  animal tissue;  Article;  controlled study;  dosimetry;  down regulation;  electric field;  electromagnetic radiation;  gene expression;  gene ontology;  hippocampus;  male;  mass fragmentography;  microarray analysis;  mouse;  nonhuman;  priority journal;  radiation exposure;  real time polymerase chain reaction;  signal transduction;  spectrophotometry;  thin layer chromatography;  transesterification;  upregulation;  analogs and derivatives;  animal;  brain;  C57BL mouse;  cell communication;  lipid metabolism;  metabolism;  mobile phone;  physiology;  radiation response;  radiofrequency radiation;  radiometry, Animals;  Brain;  Cell Communication;  Cell Phone;  Eicosapentaenoic Acid;  Fatty Acids;  Fatty Acids, Monounsaturated;  Hippocampus;  Lipid Metabolism;  Male;  Mice, Inbred C57BL;  Radio Waves;  Radiometry;  Signal Transduction;  Transcriptome",
    abstract = "Background: The widespread use of wireless devices during the last decades is raising concerns about adverse health effects of the radiofrequency electromagnetic radiation (RF-EMR) emitted from these devices. Recent research is focusing on unraveling the underlying mechanisms of RF-EMR and potential cellular targets. The “omics” high-throughput approaches are powerful tools to investigate the global effects of RF-EMR on cellular physiology. Methods: In this work, C57BL/6 adult male mice were whole-body exposed (n E xp  = 8) for 2 hr to GSM 1800 MHz mobile phone radiation at an average electric field intensity range of 4.3–17.5 V/m or sham-exposed (n SE  = 8), and the RF-EMR effects on the hippocampal lipidome and transcriptome profiles were assessed 6 hr later. Results: The data analysis of the phospholipid fatty acid residues revealed that the levels of four fatty acids [16:0, 16:1 (6c + 7c), 18:1 9c, eicosapentaenoic acid omega-3 (EPA, 20:5 ω3)] and the two fatty acid sums of saturated and monounsaturated fatty acids (SFA and MUFA) were significantly altered (p < 0.05) in the exposed group. The observed changes indicate a membrane remodeling response of the tissue phospholipids after nonionizing radiation exposure, reducing SFA and EPA, while increasing MUFA residues. The microarray data analysis demonstrated that the expression of 178 genes changed significantly (p < 0.05) between the two groups, revealing an impact on genes involved in critical biological processes, such as cell cycle, DNA replication and repair, cell death, cell signaling, nervous system development and function, immune system response, lipid metabolism, and carcinogenesis. Conclusions: This study provides preliminary evidence that mobile phone radiation induces hippocampal lipidome and transcriptome changes that may explain the brain proteome changes and memory deficits previously shown by our group. © 2018 The Authors. Brain and Behavior published by Wiley Periodicals, Inc."
}