@article{2999737,
    title = "An integrin αEβ7-dependent mechanism of IgA transcytosis requires direct plasma cell contact with intestinal epithelium",
    author = "Guzman, M. and Lundborg, L.R. and Yeasmin, S. and Tyler, C.J. and Zgajnar, N.R. and Taupin, V. and Dobaczewska, K. and Mikulski, Z. and Bamias, G. and Rivera-Nieves, J.",
    journal = "Mucosal Immunology",
    year = "2021",
    volume = "14",
    number = "6",
    pages = "1347-1357",
    publisher = "Springer Nature BV",
    issn = "1933-0219, 1935-3456",
    doi = "10.1038/s41385-021-00439-x",
    abstract = "Efficient IgA transcytosis is critical for the maintenance of a homeostatic microbiota. In the canonical model, locally-secreted dimeric (d)IgA reaches the polymeric immunoglobulin receptor (pIgR) on intestinal epithelium via simple diffusion. A role for integrin αE(CD103)β7 during transcytosis has not been described, nor its expression by intestinal B cell lineage cells. We found that αE-deficient (αE−/−) mice have a luminal IgA deficit, despite normal antibody-secreting cells (ASC) recruitment, local IgA production and increased pIgR expression. This deficit was not due to dendritic cell (DC)-derived retinoic acid (RA) nor class-switching defects, as stool from RAG−/− mice reconstituted with αE−/− B cells was also IgA deficient. Flow cytometric, ultrastructural and transcriptional profiling showed that αEβ7-expressing ASC represent an undescribed subset of terminally-differentiated intestinal plasma cells (PC) that establishes direct cell to cell contact with intestinal epithelium. We propose that IgA not only reaches pIgR through diffusion, but that αEβ7+ PC dock with E-cadherin-expressing intestinal epithelium to directly relay IgA for transcytosis into the intestinal lumen. © 2021, This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply."
}