@article{2999805,
    title = "Direct Antibiotic Activity of Bacillibactin Broadens the Biocontrol Range of Bacillus amyloliquefaciens MBI600",
    author = "Dimopoulou, A. and Theologidis, I. and Benaki, D. and Koukounia, M. and Zervakou, A. and Tzima, A. and Diallinas, G. and Hatzinikolaou, D.G. and Skandalis, N.",
    journal = "mSphere",
    year = "2021",
    volume = "6",
    number = "4",
    pages = "1-15",
    publisher = "American Society for Microbiology",
    issn = "2379-5042",
    doi = "10.1128/mSphere.00376-21",
    keywords = "antibiotic agent;  bacillibactin;  iron;  mbi 600;  siderophore;  unclassified drug;  antifungal agent;  antiinfective agent;  bacillibactin;  oligopeptide;  siderophore, antifungal activity;  antimicrobial activity;  Article;  Bacillus amyloliquefaciens;  bacterium identification;  bacterium isolation;  biological control agent;  biosynthesis;  controlled study;  genetic association;  in vitro study;  microbial competition;  nonhuman;  phytopathogen;  plant growth;  Pseudomonas syringae;  upregulation;  antibiosis;  Bacillus amyloliquefaciens;  biological control agent;  chemistry;  drug effect;  fungus;  genetics;  metabolism;  microbiology;  pathogenicity;  plant disease;  prevention and control, Anti-Bacterial Agents;  Antibiosis;  Antifungal Agents;  Bacillus amyloliquefaciens;  Biological Control Agents;  Fungi;  Iron;  Oligopeptides;  Plant Diseases;  Pseudomonas syringae;  Siderophores",
    abstract = "Bacillus amyloliquefaciens is considered the most successful biological control agent due to its ability to colonize the plant rhizosphere and phyllosphere where it outgrows plant pathogens by competition, antibiosis, and inducing plant defense. Its antimicrobial function is thought to depend on a diverse spectrum of secondary metabolites, including peptides, cyclic lipopeptides, and polyketides, which have been shown to target mostly fungal pathogens. In this study, we isolated and characterized the catecholate siderophore bacillibactin by B. amyloliquefaciens MBI600 under iron-limiting conditions and we further identified its potential antibiotic activity against plant pathogens. Our data show that bacillibactin production restrained in vitro and in planta growth of the nonsusceptible (to MBI600) pathogen Pseudomonas syringae pv. tomato. Notably, it was also related to increased antifungal activity of MBI600. In addition to bacillibactin biosynthesis, iron starvation led to upregulation of specific genes involved in microbial fitness and competition © 2021. Dimopoulou et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license"
}