@article{3000589,
    title = "The acetate uptake transporter family motif “NPAPLGL(M/S)” is essential for substrate uptake",
    author = "Ribas, D. and Soares-Silva, I. and Vieira, D. and Sousa-Silva, M. and Sá-Pessoa, J. and Azevedo-Silva, J. and Viegas, S.C. and Arraiano, C.M. and Diallinas, G. and Paiva, S. and Soares, P. and Casal, M.",
    journal = "Fungal Genetics and Biology",
    year = "2019",
    volume = "122",
    pages = "1-10",
    publisher = "Academic Press Inc.",
    issn = "1087-1845, 1096-0937",
    doi = "10.1016/j.fgb.2018.10.001",
    keywords = "acetic acid;  asparaginylprolylalanylprolylleucylglycylleucine;  G protein coupled receptor;  G protein coupled receptor 1;  peptides and proteins;  unclassified drug;  acetic acid;  ATO2 protein, S cerevisiae;  carrier protein;  membrane protein;  Saccharomyces cerevisiae protein;  succinic acid, Article;  carbon source;  controlled study;  enzyme activity;  enzyme substrate;  genotype;  Methanosarcina acetivorans;  molecular docking;  nonhuman;  phylogenetic tree;  phylogeny;  priority journal;  protein transport;  sequence alignment;  Yarrowia lipolytica;  amino acid sequence;  chemistry;  enzymology;  genetics;  metabolism;  Methanosarcina;  protein motif;  Saccharomyces cerevisiae;  transport at the cellular level;  Yarrowia, Acetic Acid;  Amino Acid Motifs;  Amino Acid Sequence;  Biological Transport;  Membrane Proteins;  Membrane Transport Proteins;  Methanosarcina;  Phylogeny;  Saccharomyces cerevisiae;  Saccharomyces cerevisiae Proteins;  Succinic Acid;  Yarrowia",
    abstract = "Organic acids are recognized as one of the most prevalent compounds in ecosystems, thus the transport and assimilation of these molecules represent an adaptive advantage for organisms. The AceTr family members are associated with the active transport of organic acids, namely acetate and succinate. The phylogenetic analysis shows this family is dispersed in the tree of life. However, in eukaryotes, it is almost limited to microbes, though reaching a prevalence close to 100% in fungi, with an essential role in spore development. Aiming at deepening the knowledge in this family, we studied the acetate permease AceP from Methanosarcina acetivorans, as the first functionally characterized archaeal member of this family. Furthermore, we demonstrate that the yeast Gpr1 from Yarrowia lipolytica is an acetate permease, whereas the Ady2 closest homologue in Saccharomyces cerevisiae, Fun34, has no role in acetate uptake. In this work, we describe the functional role of the AceTr conserved motif NPAPLGL(M/S). We further unveiled the role of the amino acid residues R122 and Q125 of SatP as essential for protein activity. © 2018 Elsevier Inc."
}