@article{3002791,
    title = "Type I and II interferon signatures in Sjogren's syndrome pathogenesis: Contributions in distinct clinical phenotypes and Sjogren's related lymphomagenesis",
    author = "Nezos, A. and Gravani, F. and Tassidou, A. and Kapsogeorgou, E.K. and Voulgarelis, M. and Koutsilieris, M. and Crow, M.K. and Mavragani, C.P.",
    journal = "Journal of Autoimmune Diseases",
    year = "2015",
    volume = "63",
    pages = "47-58",
    publisher = "INSTAP Academic Press",
    issn = "1740-2557",
    doi = "10.1016/j.jaut.2015.07.002",
    keywords = "alpha interferon;  B cell activating factor;  gamma interferon;  La antibody;  messenger RNA;  rheumatoid factor;  Ro antibody;  biological marker;  gamma interferon;  interferon, adolescent;  adult;  arthralgia;  arthritis;  Article;  carcinogenesis;  cholangitis;  clinical article;  concentration (parameters);  controlled study;  cryoglobulinemia;  disease duration;  dry eye;  erythrocyte sedimentation rate;  female;  gene;  gene overexpression;  glomerulonephritis;  histopathology;  human;  human tissue;  hypergammaglobulinemia;  hypocomplementemia;  interferon inducible gene;  interstitial nephritis;  leukopenia;  liver disease;  lung disease;  lymphadenopathy;  lymphocytopenia;  lymphoma;  lymphomagenesis;  male;  minor saliva gland;  peripheral neuropathy;  phenotype;  primary biliary cirrhosis;  priority journal;  purpura;  Raynaud phenomenon;  real time polymerase chain reaction;  receiver operating characteristic;  salivary gland biopsy;  salivary gland disease;  serology;  Sjoegren syndrome;  xerostomia;  blood;  lymphoma;  metabolism;  middle aged;  Sjogren's Syndrome, Adult;  Biomarkers;  Female;  Humans;  Interferon Type I;  Interferon-gamma;  Lymphoma;  Male;  Middle Aged;  Real-Time Polymerase Chain Reaction;  Salivary Glands, Minor;  Sjogren's Syndrome",
    abstract = "Both type I and II interferons (IFNs) have been implicated in the pathogenesis of Sjogren's syndrome (SS). We aimed to explore the contribution of type I and II IFN signatures in the generation of distinct SS clinical phenotypes including lymphoma development. Peripheral blood (PB) from SS patients (n = 31), SS patients complicated by lymphoma (n = 13) and healthy controls (HC, n = 30) were subjected to real-time PCR for 3 interferon inducible genes (IFIGs) preferentially induced by type I IFN, 2 IFIGs preferentially induced by IFNγ as well as for IFNα and IFNγ genes. The same analysis was performed in minor salivary gland tissues (MSG) derived from 31 SS patients, 10 SS-lymphoma patients and 17 sicca controls (SC). In PB and MSG tissues, overexpression of both type I and type II IFIGs was observed in SS patients versus HC and SC, respectively, with a predominance of type I IFN signature in PB and a type II IFN signature in MSG tissues. In SS-lymphoma MSG tissues, lower IFNα, but higher IFNγ and type II IFIG transcripts compared to both SS and SC were observed. In receiver operating characteristic curve analysis, IFNγ/IFNα mRNA ratio in MSG tissues showed the best discrimination for lymphoma development. Discrete expression patterns of type I and II IFN signatures might be related to distinct SS clinical phenotypes. Additionally, IFNγ/IFNα mRNA ratio in diagnostic salivary gland biopsies is proposed as a novel histopathological biomarker for the prediction of in situ lymphoma development in the setting of SS. © 2015 Elsevier Ltd."
}