@article{3002801,
    title = "Further studies on the biological activity of hazelnut allergens",
    author = "Blanc, F. and Bernard, H. and Ah-Leung, S. and Przybylski-Nicaise, L. and Skov, P.S. and Purohit, A. and De Blay, F. and Ballmer-Weber, B. and Fritsche, P. and Rivas, M.F. and Reig, I. and Sinaniotis, A. and Vassilopoulou, E. and Hoffmann-Sommergruber, K. and Vieths, S. and Rigby, N. and Mills, C. and Adel-Patient, K.",
    journal = "Clinical and Translational Allergy",
    year = "2015",
    volume = "5",
    number = "1",
    publisher = "BioMed Central Ltd.",
    doi = "10.1186/s13601-015-0066-7",
    keywords = "food allergen;  globulin;  hazelnut allergen;  immunoglobulin E;  unclassified drug, adolescent;  adult;  Article;  basophil;  biological activity;  child;  clinical article;  controlled study;  female;  France;  Greece;  hazelnut allergy;  histamine release;  human;  immunoblotting;  limit of detection;  limit of quantitation;  male;  middle aged;  peanut allergy;  preschool child;  priority journal;  school child;  self report;  sensitization;  Spain;  Switzerland;  young adult",
    abstract = "Background: Sensitization to hazelnut allergens vary depending on the geographic origin and age of the patients. The objective of this study was to further investigate the allergenic activity of hazelnut allergens using sera from patients recruited in various European regions and presenting different sensitization patterns to hazelnut proteins. Methods: Natural Cor a 11 and Cor a 9 were purified from hazelnut whereas Cor a 1 and Cor a 8 were produced as recombinant proteins (rCor a 1.04 and rCor a 8). Sera from hazelnut allergic patients were collected in France (n = 5), Switzerland (n = 2), Greece (n = 11) and Spain (n = 3), within the Europrevall project. Total and allergen-specific IgE were quantified by enzyme allergosorbent test and IgE immunoblot were performed using pooled sera from birch-pollen endemic region or from Greece. Histamine Release (HR) assays were performed with stripped basophils passively sensitized with individual sera and challenged by a hazelnut extract or the different hazelnut allergens. Results: As previously described, hazelnut allergic patients from Mediterranean countries are mainly sensitized to the nsLTP Cor a 8 whereas patients from France and Switzerland are sensitized to pollen-related allergens. Interestingly, an intermediate profile was evidenced in patients from Madrid. Hazelnut 7S globulin (Cor a 11) and 11S globulin (Cor a 9) were found to be minor allergens, recognized only by patients from Mediterranean countries. The biologic activity of the 4 tested allergens, analysed by HR assay, further confirmed the sensitization patterns, but also demonstrated the very high elicitation potency of Cor a 8. Conclusions: This work, extending previously published researches, represents a step towards the better understanding of the complexity of hazelnut allergy and provides new data on the biological activity of hazelnut allergens and extracts. © 2015 Blanc et al."
}