@article{3004012,
    title = "A molecular diagnostic algorithm to guide pollen immunotherapy in southern europe: Towards component-resolved management of allergic diseases",
    author = "Douladiris, N. and Savvatianos, S. and Roumpedaki, I. and Skevaki, C. and Mitsias, D. and Papadopoulos, N.G.",
    journal = "International Archives of Allergy and Immunology",
    year = "2013",
    volume = "162",
    number = "2",
    pages = "163-172",
    issn = "1018-2438, 1423-0097",
    doi = "10.1159/000353113",
    keywords = "carbohydrate;  immunoglobulin E;  allergen;  cross reactive carbohydrate determinant;  grass pollen extract;  pollen antigen;  profilin;  recombinant allergen;  unclassified drug, allergic disease;  article;  classification algorithm;  cross reaction;  immunotherapy;  molecular diagnosis;  Poaceae;  pollen;  prescription;  priority journal;  sensitization;  Southern Europe;  tree;  weed;  algorithm;  allergic disease;  Cynodon dactylon;  grass pollen;  human;  Parietaria;  patient care;  pollen allergy;  protein processing;  respiratory tract allergy;  Review, Algorithms;  Allergens;  Antigens, Plant;  Desensitization, Immunologic;  Europe;  Humans;  Immunoglobulin E;  Molecular Diagnostic Techniques;  Plant Proteins;  Pollen;  Rhinitis, Allergic, Seasonal",
    abstract = "Correct identification of the culprit allergen is an essential part of diagnosis and treatment in immunoglobulin E (IgE)-mediated allergic diseases. In recent years, molecular biology has made important advances facilitating such identification and overcoming some of the drawbacks of natural allergen extracts, which consist of mixtures of various proteins that may be allergenic or not, specific for the allergen source or widely distributed (panallergens). New technologies offer the opportunity for a more accurate component-resolved diagnosis, of benefit especially to polysensitized allergic patients. The basic elements of molecular diagnostics with potential relevance to immunotherapy prescription are reviewed here, with a focus on Southern European sensitization patterns to pollen allergens. We propose a basic algorithm regarding component-resolved diagnostic work-up for pollen allergen-specific immunotherapy candidates in Southern Europe; this and similar algorithms can form the basis of improved patient management, conceptually a 'Component-Resolved Allergy Management'. Copyright © 2013 S. Karger AG, Basel."
}