@article{3004305,
    title = "Red blood cell aging markers during storage in citrate-phosphate-dextrose- saline-adenine-glucose-mannitol",
    author = "Antonelou, M.H. and Kriebardis, A.G. and Stamoulis, K.E. and Economou-Petersen, E. and Margaritis, L.H. and Papassideri, I.S.",
    journal = "Blood Transfusion = Transfusione del Sangue",
    year = "2010",
    volume = "50",
    number = "2",
    pages = "376-389",
    issn = "1723-2007, 2385-2070",
    doi = "10.1111/j.1537-2995.2009.02449.x",
    keywords = "adenine;  biological marker;  caspase;  caspase 3;  caspase 8;  cathepsin E;  citric acid;  erythrocyte band 3 protein;  Fas antigen;  Fas associated death domain protein;  flotillin 1;  flotillin 2;  glucose;  heat shock protein 27;  heat shock protein 70;  hemoglobin;  immunoglobulin G;  mannitol;  peroxiredoxin 2;  phosphate;  procaspase 3;  sodium chloride;  sorcin;  spectrin;  stomatin;  synexin;  ubiquitin, article;  cell vacuole;  clinical article;  controlled study;  enzyme activation;  erythrocyte lifespan;  erythrocyte preservation;  exocytosis;  human;  human cell;  immunoblotting;  immunoelectron microscopy;  immunofluorescence;  immunogold labeling;  oligomerization;  oxidation;  oxidative stress;  polyacrylamide gel electrophoresis;  protein aggregation;  protein carbonylation;  protein cleavage;  protein degradation;  protein expression;  protein localization;  Western blotting, Adenine;  Adult;  Anticoagulants;  Biological Markers;  Blood Preservation;  Blood Proteins;  Citrates;  Cryoprotective Agents;  Enzyme Activation;  Erythrocyte Aging;  Erythrocytes;  Exocytosis;  Free Radical Scavengers;  Glucose;  Humans;  Mannitol;  Membrane Microdomains;  Microscopy, Immunoelectron;  Oxidation-Reduction;  Phosphates;  Protein Carbonylation;  Protein Stability;  Sodium Chloride",
    abstract = "Background: It has been suggested that red blood cell (RBC) senescence is accelerated under blood bank conditions, although neither protein profile of RBC aging nor the impact of additive solutions on it have been studied in detail. Study Design and Methods: RBCs and vesicles derived from RBCs in both citrate-phosphate-dextrose (CPD)-saline-adenine-glucose-mannitol (SAGM) and citrate-phosphate-dextrose-adenine (CPDA) were evaluated for the expression of cell senescence markers (vesiculation, protein aggregation, degradation, activation, oxidation, and topology) through immunoblotting technique and immunofluorescence or immunoelectron microscopy study. Results: A group of cellular stress proteins exhibited storage time- and storage medium-related changes in their membrane association and exocytosis. The extent, the rate, and the expression of protein oxidation, Fas oligomerization, caspase activation, and protein modifications in Band 3, hemoglobin, and immunoglobulin G were less conspicuous and/or exhibited significant time retardation under storage in CPD-SAGM, compared to the CPDA storage. There was evidence for the localization of activated caspases near to the membrane of both cells and vesicles. ConclusionS: We provide circumstantial evidence for a lower protein oxidative damage in CPD-SAGM-stored RBCs compared to the CPDA-stored cells. The different expression patterns of the senescence markers in the RBCs seem to be accordingly related to the oxidative stress management of the cells. We suggest that the storage of RBCs in CPD-SAGM might be more alike the in vivo RBC aging process, compared to storage in CPDA, since it is characterized by a slower stimulation of the recognition signaling pathways that are already known to trigger the erythrophagocytosis of senescent RBCs. © 2009 American Association of Blood Banks."
}