@article{3005079,
    title = "A radioimmunoassay for parathymosin α using antibodies to synthetic N-terminal peptide 1-30",
    author = "Tsitsiloni, O.E. and Yialouris, P.P. and Heimer, E.P. and Felix, A.M. and Evangelatos, G.P. and Soteriadis-Vlahos, C. and Stiakakis, J. and Hannappel, E. and Haritos, A.A.",
    journal = "Journal of Immunological Methods",
    year = "1988",
    volume = "113",
    number = "2",
    pages = "175-184",
    issn = "0022-1759",
    doi = "10.1016/0022-1759(88)90330-4",
    keywords = "epitope;  radioisotope;  synthetic peptide;  thymosin, animal cell;  animal experiment;  cattle;  cell culture;  human;  human cell;  methodology;  nonhuman;  priority journal;  rabbit;  radioimmunoassay;  rat, Adult;  Aged;  Amino Acid Sequence;  Animal;  Cattle;  Chromatography, High Pressure Liquid;  Cross Reactions;  Female;  Human;  Immune Sera;  Infant;  Molecular Sequence Data;  Peptide Fragments;  Peptide Hydrolases;  Rabbits;  Radioimmunoassay;  Support, Non-U.S. Gov't;  Thymosin;  Tissue Distribution",
    abstract = "Antibodies against the N-terminus of rat parathymosin α have been raised in rabbits by conjugating parathymosin α (1-30) to hemocyanin. A radioimmunoassay for parathymosin α was established by utilizing antibodies against the above polypeptide and parathymosin α(1-12)[Tyr] as tracer. The useful range was 5-450 pmol for parathymosin α. An epitope was located in the amino acid sequence 1-12. The antiserum failed to crossreact with the same molar concentrations of the partly homologous thymosin α1 or prothymosin α. With this radioimmunoassay, parathymosin α was isolated from calf thymus after separation from prothymosin α by reversed phase HPLC. Endogenous proteases did not appear to generate N-terminal fragments of parathymosin α in rat liver extracts in a similar fashion to that observed for prothymosin α. Parathymosin α has a ubiquitous distribution in the human tissues examined, with levels ranging from 93 (brain) to 1043 (liver) ng of parathymosin α(1-30) equivalents/g (wet weight). © 1988."
}