@article{3015262,
    title = "The expression of Omental 11β-HSD1 is not increased in severely obese women with metabolic syndrome",
    author = "Michalaki, M. and Kyriazopoulou, V. and Antonacopoulou, A. and Koika, V. and Nikolaou, M. and Tsoukas, A. and Kalfarentzos, F. and Vagenakis, A.G. and Voukelatou, G. and Papavassiliou, A.G.",
    journal = "Obesity Facts",
    year = "2012",
    volume = "5",
    number = "1",
    pages = "104-111",
    issn = "1662-4025, 1662-4033",
    doi = "10.1159/000336755",
    keywords = "11beta hydroxysteroid dehydrogenase 1;  glucocorticoid receptor alpha;  glucocorticoid receptor beta;  metformin, adult;  article;  bariatric surgery;  clinical article;  controlled study;  disease severity;  female;  human;  human tissue;  hypercortisolism;  hysterectomy;  metabolic syndrome X;  non insulin dependent diabetes mellitus;  obesity;  peritoneal biopsy;  priority journal;  protein expression;  real time polymerase chain reaction;  subcutaneous fat, 11-beta-Hydroxysteroid Dehydrogenase Type 1;  Adult;  Analysis of Variance;  Bariatric Surgery;  Biopsy;  Female;  Humans;  Hydrocortisone;  Hysterectomy;  Intra-Abdominal Fat;  Metabolic Syndrome X;  Middle Aged;  Obesity, Morbid;  Real-Time Polymerase Chain Reaction;  Receptors, Glucocorticoid;  RNA, Messenger;  Subcutaneous Fat;  Young Adult",
    abstract = "Objective: Plasma cortisol in obese subjects does not differ from that in normoweight subjects. Extra-adrenal cortisol production by 11β- hydroxysteroid dehydrogenase type 1 (11β-HSD1) can result in local hypercortisolemia. The aim of the present study was to examine the role of visceral hypercortisolemia in the development of metabolic syndrome in severe obesity. Methods: Eight lean women during hysterectomy (controls) and 19 severely obese women during bariatric surgery were studied, 8 without metabolic syndrome (OM- group) and 11 with it (OM+ group). Biopsies of omental and subcutaneous fat were performed in the severely obese women during surgery, but only omental biopsies in the controls. Expression of 11β-HSD1, glucocorticoid receptor α (GRα) and glucocorticoid receptor β (GRβ) was evaluated using real-time PCR.Results: Omental 11β-HSD1 expression was different between groups (one-way ANOVA, p < 0.01). Post-hoc analysis revealed that mean omental 11β-HSD1 mRNA levels were higher in the OM- group compared to controls, whereas they were similar when comparing the OM+ group with lean controls. Expression of 11β-HSD1 in subcutaneous fat was not different between OM+ and OM- groups. GRα expression in omental fat did not differ among groups or between omental and subcutaneous fat in severely obese patients. An expression of GRβ was not detected.Conclusion: Contrary to our original hypothesis, omental 11β-HSD1 expression is not increased in the OM+ group. Copyright © 2012 S. Karger GmbH, Freiburg."
}