@article{3020746,
    title = "Preliminary data of a quantitative point of care test for SARS-CoV-2 antibodies from greece",
    author = "FRAGKOU, P.C. and PAPAEVANGELOU, V. and ANTONIADOU, A. and KAVVATHA, D. and PLOUSSI, A. and PANTAZIS, N. and SIRMPILANTZE, T. and PSARRAKIS, C. and POURNARAS, S.A. and TSIODRAS, S. and KELEKIS, A.",
    journal = "In vivo (Athens, Greece)",
    year = "2020",
    volume = "34",
    number = "5",
    pages = "3039-3045",
    publisher = "International Institute of Anticancer Research",
    doi = "10.21873/invivo.12138",
    keywords = "immunoglobulin G;  immunoglobulin M;  SARS-CoV-2 antibody;  virus RNA;  immunoglobulin G;  virus antibody, aged;  Article;  clinical article;  controlled study;  coronavirus disease 2019;  diagnostic test accuracy study;  disease severity;  female;  fluorescence;  follow up;  Greece;  human;  immunoassay;  male;  observational study;  point of care testing;  preliminary data;  prospective study;  quantitative diagnosis;  real time reverse transcription polymerase chain reaction;  RNA extraction;  sensitivity and specificity;  Severe acute respiratory syndrome coronavirus 2;  Betacoronavirus;  blood;  Coronavirus infection;  isolation and purification;  middle aged;  pandemic;  pathogenicity;  point of care system;  virology;  virus pneumonia, Aged;  Antibodies, Viral;  Betacoronavirus;  Coronavirus Infections;  Greece;  Humans;  Immunoglobulin G;  Male;  Middle Aged;  Pandemics;  Pneumonia, Viral;  Point-of-Care Systems;  Sensitivity and Specificity",
    abstract = "Background: Antibody testing is necessary to identify immune individuals in the post-initial wave of the COVID-19 pandemic. Patients and Methods: We prospectively evaluated the performance of a quantitative point-of-care test (POCT) for SARS-CoV-2 antibodies. The patient group (PG) comprised of hospitalized confirmed COVID-19 cases. Asymptomatic healthcare volunteers with negative rRT-PCR were included in the control group (CG). Measurement of IgM and IgG was obtained by dry fluorescence immunoassay. Results: Twenty-six PG (65.9±15.4 years old, male 57.7%) and 18 CG (45.6±10.1 years old, male 33.3%) were included. By manufacturer's cut-off (≥0.04 mIU/ml), sensitivity and specificity were 73.08% and 88.89% for IgM and 88.46% and 33.33% for IgG, respectively. Estimated areas under the ROC curve were 0.907 and 0.848 for IgM and IgG, respectively. Results were improved using a cut-off of IgM ≥0.05 mIU/ml and IgG ≥0.10 mIU/ml. Conclusion: Using stringent cut-off values, SARS-CoV-2 antibody POCT detects immune people and can be used during socioeconomic normalization of communities. © 2020 International Institute of Anticancer Research. All rights reserved."
}