@article{3020752,
    title = "Impact of deserosalization on small bowel anastomosis healing in swine: A pilot study",
    author = "Vrakopoulou, G.-Z. and Toutouzas, K.G. and Giannios, P. and Panousopoulos, S.-G. and Theodoropoulos, C. and Danas, E. and Liakea, A. and Papalois, A.E. and Zografos, G. and Larentzakis, A.",
    journal = "In vivo (Athens, Greece)",
    year = "2020",
    volume = "34",
    number = "5",
    pages = "2423-2429",
    publisher = "International Institute of Anticancer Research",
    doi = "10.21873/invivo.12056",
    keywords = "hydroxyproline;  collagen, animal experiment;  animal model;  animal tissue;  Article;  chemical reaction;  controlled study;  deserosalization;  feasibility study;  female;  histopathology;  intestine anastomosis;  jejuno jejunal side to side anastomosis;  jejunum;  Landrace pig;  nonhuman;  pilot study;  serosa;  statistically significant result;  wound healing;  anastomosis;  animal;  colon;  pig;  small intestine;  surgery, Anastomosis, Surgical;  Animals;  Collagen;  Colon;  Intestine, Small;  Pilot Projects;  Swine;  Wound Healing",
    abstract = "Background: Healing is related to gastrointestinal anastomotic leak, which is a severe and common complication. This study aimed to investigate the feasibility and the impact of deserosalization on healing of jejuno-jejunal anastomoses in an animal model. Materials and Methods: Seven swine underwent three types of side-to-side jejuno-jejunal anastomosis twice and survived seven days. Three different types of jejuno-jejunal sideto- side anastomoses were performed twice at 20-cm distance from each other in each animal: no serosa removal, one-sided, and two-sided serosa removal, respectively. Bursting pressure, tissue hydroxyproline concentration, and pathology scores were evaluated. Results: Hydroxyproline tissue concentration was a mean±standard deviation of 0.37±0.09, 0.38±0.08, and 0.30±0.05 nmoI/ml respectively (p<0.05). Bursting pressure was a mean±standard deviation of 59.02±8.60, 73.20±11.09, and 100.01±7.49 mmHg, respectively (p<0.001). The histopathological assessment did not find any statistically significant differences. Conclusion: Deserosalization in jejunojejunal anastomosis was technically feasible and seemed to improve mechanical strength and collagen deposition in this experimental porcine model. Further investigation is warranted. © 2020 International Institute of Anticancer Research. All rights reserved."
}