@article{3021301,
    title = "The GnRH analogues affect novel neuropeptide SMIM20/phoenixin and GPR173 receptor expressions in the female rat hypothalamic–pituitary–gonadal (HPG) axis",
    author = "Suszka-Świtek, A. and Pałasz, A. and Filipczyk, Ł. and Menezes, I.C. and Mordecka-Chamera, K. and Angelone, T. and Bogus, K. and Bacopoulou, F. and Worthington, J.J. and Wiaderkiewicz, R.",
    journal = "Clinical and Experimental Pharmacology and Physiology",
    year = "2011",
    volume = "46",
    number = "4",
    pages = "350-359",
    publisher = "Blackwell Publishing Inc.",
    issn = "0305-1870, 1440-1681",
    doi = "10.1111/1440-1681.13061",
    keywords = "buserelin;  cetrorelix;  gonadorelin derivative;  gpr173 receptor;  messenger RNA;  neuropeptide;  neuropeptide receptor;  phoenixin protein;  unclassified drug, adult;  animal experiment;  Article;  controlled study;  diestrus;  drug megadose;  enzyme linked immunosorbent assay;  female;  gene expression;  hypothalamus hypophysis gonad system;  nonhuman;  ovary;  proestrus;  protein blood level;  protein expression;  quantitative analysis;  rat;  real time polymerase chain reaction;  reproduction;  signal transduction",
    abstract = "The recently discovered peptide phoenixin (PNX) and its receptor GPR173 are novel factors that exhibit a large spectrum of regulatory activity, especially when considered as a central modulator of GnRH-related hormonal control of reproductive processes. It has been already proven that GnRH agonists and antagonists can modulate peptidergic signalling in the HPG axis. Despite these findings, there is so far no information regarding the influence of treatment with GnRH analogues on SMIM20/phoenixin signalling in the hypothalamic–pituitary–gonadal axis. In the current study, SMIM20/phoenixin and GPR173 mRNA levels were measured in the hypothalamus, pituitary and ovaries of female rats in the dioestrus phase following treatment with GnRH-R agonist (buserelin) and antagonist (cetrorelix) using quantitative real-time PCR. The serum PNX concentrations were also estimated with ELISA technique. The hypothalamic, hypophyseal and especially ovarian levels of SMIM20 mRNA were increased after both buserelin and cetrorelix administration. The GPR173 expressions were in turn decreased in the hypothalamus and pituitary. Treatment with the GnRH analogues led to the modulation of SMIM20/phoenixin and GPR173 mRNA expression in the female rat hypothalamic–pituitary–gonadal axis. By identifying buserelin and cetrorelix as novel modulators of phoenixin signalling in the animal HPG axis, these results cast new light on the GnRH analogues mode of action and contribute to a better understanding of the mechanisms responsible for the hormonal control of reproduction. © 2019 John Wiley & Sons Australia, Ltd"
}