@article{3025605,
    title = "Antithrombotic and antiplatelet activity of an organometallic rhodium(I) complex incorporating a substituted thieno-[2,3-d]-pyrimidine ligand: Synthesis, structural characterization, and molecular docking calculations",
    author = "Kalampalidis, A. and Peppas, A. and Schnakenburg, G. and Papakyriakou, A. and Tsoupras, A. and Zabetakis, I. and Philippopoulos, A.I.",
    journal = "Applied Organometallic Chemistry",
    year = "2021",
    volume = "35",
    number = "6",
    publisher = "John Wiley and Sons Ltd",
    issn = "0268-2605, 1099-0739",
    doi = "10.1002/aoc.6210",
    keywords = "Aromatic compounds;  Carboxylation;  Chelation;  Geometry;  Ligands;  Molecular modeling;  Nuclear magnetic resonance spectroscopy;  Organometallics;  Phospholipids;  Platelets;  Rhodium compounds;  Single crystals;  Spectroscopic analysis, Anti-inflammatory activity;  Anti-platelet activities;  Anti-thrombotic activities;  Platelet-activating factors;  Single crystal X-ray crystallography;  Spectroscopic technique;  Square planar geometry;  Structural characterization, X ray crystallography",
    abstract = "The antiplatelet and antithrombotic activity of a novel organometallic rhodium(I) complex of the formula [Rh(cod)Cl(tpc)] (1) (cod = cis-1,5-cyclooctadiene; tpc = methyl 2-amino-4-(diethylamino)-thieno-[2,3-d]-pyrimidine-6-carboxylate) was investigated. Complex 1 was easily synthesized by a one-pot, high-yield reaction and was fully characterized by standard spectroscopic techniques including FT-IR, UV–Vis, and NMR spectroscopy and by elemental analysis. The molecular structures of tpc and 1 were determined by single-crystal X-ray crystallography. Complex 1 displayed a slightly distorted square planar geometry and is the first crystallographically characterized example of a coordination compound bearing the ligand precursor tpc. Biological studies demonstrate that 1 displays strong antiplatelet and antithrombotic properties in vitro, by inhibiting both the aggregation of human and washed rabbit platelets induced by the potent inflammatory and thrombotic mediator, platelet-activating factor (PAF) in the micromolar range. This is an approach of continuous interest in the field. Molecular docking calculations suggest that 1 can fit at the ligand-binding site of the PAF receptor (PAFR) and thus block PAF thrombotic activities, through an antagonistic effect on the PAF/PAFR-related pathway, which is in accord with the experimental findings. Complex 1 constitutes an interesting example of a metal-based PAF inhibitor with promising antiplatelet, antithrombotic, and anti-inflammatory activity, because PAF is the most potent inflammatory lipid mediator. This is also supported by the fact that 1 is an inhibitor of other inflammatory and thrombotic mediators like thrombin, along with well-established platelet agonists like ADP and collagen. © 2021 John Wiley & Sons, Ltd."
}