@article{3026068,
    title = "A selective and sensitive method for quantification of endogenous polysulfide production in biological samples",
    author = "Bibli, S.-I. and Luck, B. and Zukunft, S. and Wittig, J. and Chen, W. and Xian, M. and Papapetropoulos, A. and Hu, J. and Fleming, I.",
    journal = "Redox Biology",
    year = "2018",
    volume = "18",
    pages = "295-304",
    publisher = "Elsevier B.V.",
    issn = "2213-2317",
    doi = "10.1016/j.redox.2018.07.016",
    keywords = "cystathionine gamma lyase;  fluorescent dye;  hydrogen sulfide;  polysulfide;  sulfide;  unclassified drug;  cystathionine gamma lyase;  polysulfide;  sulfide, animal cell;  animal experiment;  animal tissue;  Article;  biological activity;  biological model;  complex formation;  controlled study;  endothelium cell;  enzyme activity;  female;  heart;  human;  human cell;  limit of detection;  liquid chromatography-mass spectrometry;  male;  mouse;  nonhuman;  priority journal;  regulatory mechanism;  animal;  C57BL mouse;  cardiac muscle;  cell culture;  chemistry;  enzymology;  liquid chromatography;  metabolism;  procedures;  tandem mass spectrometry, Animals;  Cells, Cultured;  Chromatography, Liquid;  Cystathionine gamma-Lyase;  Endothelial Cells;  Humans;  Mice, Inbred C57BL;  Myocardium;  Sulfides;  Tandem Mass Spectrometry",
    abstract = "Hydrogen sulfide (H2S) is a gasotransmitter that regulates cellular homeostasis and impacts on multiple physiological and pathophysiological processes. However, it exerts many of its biological actions indirectly via the formation of H2S-derived sulfane sulfur species/polysulfides. Because of the high reactivity of sulfur species, the detection of H2S-derived polysulfides in biological systems is challenging and currently used methods are neither sensitive nor quantitative. Herein, we describe a LC-MS/MS-based method that makes use of Sulfane Sulfur Probe 4 to detect endogenously generated polysulfides in biological samples in a selective, sensitive and quantitative manner. The results indicate a large variability in the activity of the H2S-generating enzymes in different murine organs, but the method described was able to detect intracellular levels of polysulfides in the nanomolar range and identify cystathionine γ-lyase as the major intracellular source of sulfane sulfur species/polysulfides in murine endothelial cells and hearts. The protocol described can be applied to a variety of biological samples for the quantification of the H2S-derived polysulfides and has the potential to increase understanding on the control and consequences of this gaseous transmitter. © 2018 The Authors"
}