@article{3026742,
    title = "Pre-Column Derivatization HPLC Procedure for the Quantitation of Aluminium Chlorohydrate in Antiperspirant Creams Using Quercetin as Chromogenic Reagent",
    author = "Kalogria, E. and Varvaresou, A. and Papageorgiou, S. and Protopapa, E. and Tsaknis, I. and Matikas, A. and Panderi, I.",
    journal = "Chromatographia",
    year = "2014",
    volume = "77",
    number = "19-20",
    pages = "1275-1281",
    publisher = "Friedr. Vieweg und Sohn Verlags GmbH",
    issn = "0009-5893, 1612-1112",
    doi = "10.1007/s10337-014-2722-9",
    keywords = "acetonitrile;  aluminum hydroxide;  aluminum hydroxychloride;  ammonium acetate;  antiperspirant agent;  buffer;  methanol;  quercetin;  trifluoroacetic acid, accuracy;  Article;  complex formation;  cosmetic industry;  cream;  derivatization;  high performance liquid chromatography;  limit of detection;  limit of quantitation;  particle size;  pH;  pre column derivatization high performance liquid chromatography;  water content",
    abstract = "This article describes the development and validation of a selective high-performance liquid chromatography method that allows, after liquid–liquid extraction and pre-column derivatization reaction with quercetin, the quantification of aluminium chlorohydrate in antiperspirant creams. Chromatographic separation was achieved on an XTerra MS C18 analytical column (150 × 3.0 mm i.d., particle size 5 μm) using a mobile phase of acetonitrile:water (15:85, v/v) containing 0.08 % trifluoroacetic acid at a flow rate of 0.30 mL min−1. Ultraviolet spectrophotometric detection at 415 nm was used. The assay was linear over a concentration range of 3.7–30.6 μg mL−1 for aluminium with a limit of quantitation of 3.74 μg mL−1. Quality control samples (4.4, 17.1 and 30.6 μg mL−1) in five replicates from five different runs of analysis demonstrated intra-assay precision (% coefficient of variation <3.8 %), inter-assay precision (% coefficient of variation <5.4 %) and an overall accuracy (% recovery) between 96 and 101 %. The method was used to quantify aluminium in antiperspirant creams containing 11.0, 13.0 and 16.0 % (w/w) aluminium chlorohydrate, respectively. © 2014, The Author(s)."
}