@article{3059358,
    title = "Uptake of BSA-FITC loaded PLGA nanoparticles by bone marrow-derived dendritic cells induces maturation but not IL-12 or IL-10 production",
    author = "Karagouni, E. and Kammona, O. and Margaroni, M. and Kotti, K. and Karageorgiou, V. and Gaitanaki, C. and Kiparissides, C.",
    journal = "Nanoscience and Nanotechnology Letters",
    year = "2013",
    volume = "5",
    number = "4",
    pages = "498-504",
    issn = "1941-4900, 1941-4919",
    doi = "10.1166/nnl.2013.1564",
    keywords = "BSA-FITC;  DCs maturation;  IL-10;  IL-12;  PLGA nanoparticles, Bone;  Cells;  Copolymers;  Cytology;  Nanoparticles, Synthesis (chemical)",
    abstract = "Nanoparticles prepared from biodegradable polymers, such as poly(lactide-co-glycolide) (PLGA), represent a new approach for vaccine delivery due to their ability to be taken up by phagocytes and activate immune responses. In this study, fluorescently labelled bovine serum albumin (BSA-FITC)-loaded PLGA nanoparticles, of an average size ∼300 nm were prepared and examined for their ability to be taken up by bone marrow-derived dendritic cells (BM-DCs) in vitro and thus to promote their maturation and activation. The synthesized nanoparticles did not exhibit any cytotoxic or hemolytic effect and were taken up by BM-DCs efficiently, in a time and dose dependent manner. The localization of BSA-FITC loaded PLGA nanoparticles both in the acidophilic cellular compartments and the cytoplasm resulted in the maturation of BM-DCs expressing higher levels of costimulatory and MHC class II molecules in comparison to empty PLGA nanoparticles. However, the absence of IL-12 or IL-10 production indicates partial activation of BM-DCs suggesting the necessity of an adjuvant addition in order to facilitate DCs functionalization. Copyright © 2013 American Scientific Publishers. All rights reserved."
}