@article{3060721,
    title = "Multicenter Study of Epidemiological Cutoff Values and Detection of Resistance in Candida spp. to Anidulafungin, Caspofungin, and Micafungin Using the Sensititre YeastOne Colorimetric Method",
    author = "Espinel-Ingroff, A. and Alvarez-Fernandez, M. and Cantón, E. and Carver, P.L. and Chen, S.C.-A. and Eschenauer, G. and Getsinger, D.L. and Gonzalez, G.M. and Govender, N.P. and Grancini, A. and Hanson, K.E. and Kidd, S.E. and Klinker, K. and Kubin, C.J. and Kus, J.V. and Lockhart, S.R. and Meletiadis, J. and Morris, A.J. and Pelaez, T. and Quindós, G. and Rodriguez-Iglesias, M. and Sánchez-Reus, F. and Shoham, S. and Wengenack, N.L. and Borrell Solé, N. and Echeverria, J. and Esperalba, J. and De La Pedrosa, E.G.-G. and García García, I. and Linares, M.J. and Marco, F. and Merino, P. and Pemán, J. and Pérez Del Molino, L. and Roselló Mayans, E. and Rubio Calvo, C. and Ruiz Pérez De Pipaon, M. and Yagüe, G. and Garcia-Effron, G. and Guinea, J. and Perlin, D.S. and Sanguinetti, M. and Shields, R. and Turnidge, J.",
    journal = "Antimicrobial Agents and Chemotherapy",
    year = "2015",
    volume = "59",
    number = "11",
    pages = "6725-6732",
    publisher = "American Society for Microbiology",
    issn = "0066-4804, 1098-6596",
    doi = "10.1128/AAC.01250-15",
    keywords = "anidulafungin;  caspofungin;  micafungin;  anidulafungin;  antifungal agent;  caspofungin;  echinocandin;  lipopeptide;  micafungin, antifungal resistance;  antifungal susceptibility;  Article;  Australia;  Canada;  Candida;  Candida albicans;  Candida dubliniensis;  Candida glabrata;  Candida parapsilosis;  Candida tropicalis;  Clavispora lusitaniae;  colorimetry;  Europe;  fungal detection;  Mexico;  Meyerozyma guilliermondii;  minimum inhibitory concentration;  mutation;  New Zealand;  nonhuman;  Pichia kudriavzevii;  priority journal;  South Africa;  United States;  Candida;  drug effects;  genetics;  microbial sensitivity test, Antifungal Agents;  Candida;  Echinocandins;  Lipopeptides;  Microbial Sensitivity Tests;  Mutation",
    abstract = "Neither breakpoints (BPs) nor epidemiological cutoff values (ECVs) have been established for Candida spp. with anidulafungin, caspofungin, and micafungin when using the Sensititre YeastOne (SYO) broth dilution colorimetric method. In addition, reference caspofungin MICs have so far proven to be unreliable. Candida species wild-type (WT) MIC distributions (for microorganisms in a species/drug combination with no detectable phenotypic resistance) were established for 6,007 Candida albicans, 186 C. dubliniensis, 3,188 C. glabrata complex, 119 C. guilliermondii, 493 C. krusei, 205 C. lusitaniae, 3,136 C. parapsilosis complex, and 1,016 C. tropicalis isolates. SYO MIC data gathered from 38 laboratories in Australia, Canada, Europe, Mexico, New Zealand, South Africa, and the United States were pooled to statistically define SYO ECVs. ECVs for anidulafungin, caspofungin, and micafungin encompassing >97.5% of the statistically modeled population were, respectively, 0.12,0.25, and 0.06 μg/ml for C. albicans, 0.12, 0.25, and 0.03 μg/ml for C. glabrata complex, 4, 2, and 4 μg/ml for C. parapsilosis complex, 0.5,0.25, and 0.06 μg/ml for C. tropicalis, 0.25,1, and 0.25 μg/ml for C. krusei, 0.25,1, and 0.12 μg/ml for C. lusitaniae, 4,2, and 2 μg/ml for C. guilliermondii, and 0.25,0.25, and 0.12 μg/ml for C. dubliniensis. Species-specific SYO ECVs for anidulafungin, caspofungin, and micafungin correctly classified 72 (88.9%), 74 (91.4%), 76 (93.8%), respectively, of 81 Candida isolates with identified fks mutations. SYO ECVs may aid in detecting non-WT isolates with reduced susceptibility to anidulafungin, micafungin, and especially caspofungin, since testing the susceptibilities of Candida spp. to caspofungin by reference methodologies is not recommended. Copyright © 2015, American Society for Microbiology. All Rights Reserved."
}