@article{3062411,
    title = "Acute administration of vitamin C abrogates protection from ischemic preconditioning in rabbits",
    author = "Tsovolas, K. and Iliodromitis, E.K. and Andreadou, I. and Zoga, A. and Demopoulou, M. and Iliodromitis, K.E. and Manolaki, T. and Markantonis, S.L. and Kremastinos, D.Th.",
    journal = "Pharmacological Research Communications",
    year = "2008",
    volume = "57",
    number = "4",
    pages = "283-289",
    doi = "10.1016/j.phrs.2008.02.003",
    keywords = "ascorbic acid;  malonaldehyde;  superoxide dismutase, acute drug administration;  animal experiment;  animal model;  animal tissue;  antioxidant activity;  article;  blood sampling;  controlled study;  drug effect;  enzyme activity;  enzyme blood level;  heart infarction size;  heart muscle ischemia;  heart muscle reperfusion;  heart protection;  ischemic preconditioning;  lipid peroxidation;  male;  nonhuman;  oxidative stress;  priority journal;  rabbit, Animals;  Ascorbic Acid;  Ischemic Preconditioning, Myocardial;  Lipid Peroxidation;  Male;  Malondialdehyde;  Myocardial Infarction;  Oxidative Stress;  Rabbits;  Superoxide Dismutase",
    abstract = "Vitamin C is considered to be an antioxidant agent that is broadly used. Free radicals are involved in the protective mechanism of preconditioning (PC), but some antioxidant compounds abolish this benefit. The aim of the present study was to evaluate the effect of vitamin C on the protective effect of PC with respect to infarct size and oxidative stress in anesthetized rabbits. Male rabbits were randomly divided into six groups and subjected to 30 min of myocardial ischemia and 3 h of reperfusion with the following interventions per group: (1) Control (no intervention), (2) Vit C 150 group (i.v. vitamin C at a total dose of 150 mg/kg for 75 min, starting 40 min before the onset of long ischemia and lasting up to the 5th min of reperfusion), (3) Vit C 300 group (i.v. vitamin C at a total dose of 300 mg/kg as previously described), (4) PC group (two cycles of 5 min ischemia and 10 min reperfusion), (5) combined PC-Vit C 150 group and (6) combined PC-Vit C 300 group. Blood samples were taken at different time points for malondialdehyde (MDA) assessment as a lipid peroxidation marker and for superoxide dismutase (SOD) activity. At the end of the experiment the infarct size was determined. Vitamin C, at both doses, did not reduce the infarct size (35.5 ± 4.1%, 38.3 ± 7.0% vs. 44.9 ± 3.3% in the control group) and diminished the protection afforded by PC (32.0 ± 2.7%, 43.8 ± 3.3% vs. 15.7 ± 2.9% in the PC group, P < 0.05). At reperfusion there was an elevation of circulating MDA levels in the control and PC groups while in both vitamin C groups the levels were decreased. SOD activity was enhanced in the PC group compared to the controls; vitamin C did not change SOD activity during ischemia-reperfusion. Vitamin C abrogates the beneficial effect of ischemic PC on infarct size and elicits antioxidant properties during ischemia-reperfusion. © 2008 Elsevier Ltd. All rights reserved."
}