@article{3062854,
    title = "Development and validation of a simple reversed-phase high-performance liquid chromatography method for the determination of testosterone in serum of males",
    author = "Loi, V. and Vertzoni, M. and Vryonidou, A. and Phenekos, C.",
    journal = "Journal of Pharmaceutical and Biomedical Analysis",
    year = "2006",
    volume = "41",
    number = "2",
    pages = "527-532",
    issn = "0731-7085",
    doi = "10.1016/j.jpba.2005.11.021",
    keywords = "acetonitrile;  propyl paraben;  testosterone;  water, adult;  animal experiment;  article;  blood analysis;  calibration;  concentration (parameters);  concentration response;  controlled study;  correlation analysis;  data analysis;  elution;  female;  flow rate;  human;  liquid liquid extraction;  male;  nonhuman;  normal human;  priority journal;  quantitative analysis;  radioimmunoassay;  reversed phase high performance liquid chromatography;  testosterone blood level;  validation process;  validation study, Acetonitriles;  Chromatography, High Pressure Liquid;  Humans;  Male;  Reproducibility of Results;  Spectrophotometry, Ultraviolet;  Testosterone;  Time Factors;  Water",
    abstract = "An isocratic high-performance liquid chromatographic method with detection at 234 nm was developed, optimized and validated for the determination of testosterone in human serum. Propylparaben was used as internal standard. A Hypersil BDS RP-C18 column (150 mm × 4.6 mm, 5 μm), was equilibrated with a mobile phase composed of acetonitrile and water (35:65, v/v) and having a flow rate of 1 ml/min. The elution time for testosterone and internal standard was approximately 11.6 and 9.9 min, respectively. Calibration curves of testosterone in serum were linear in the concentration range of 1-20 ng/ml. Limits of detection and quantification in serum were 0.4 and 1.1 ng/ml, respectively. Recovery was greater than 92%. Intra- and inter-day relative standard deviation for testosterone in serum was less than 2.1 and 3.9%, respectively. This method was applied to the determination of testosterone serum levels of 12 healthy males and data were correlated with data obtained using a radioimmunoassay method. © 2005 Elsevier B.V. All rights reserved."
}