@article{3076753,
    title = "Biological effect of silver-modified nanostructured titanium dioxide in cancer",
    author = "Lagopati, N. and Kotsinas, A. and Veroutis, D. and Evangelou, K. and Papaspyropoulos, A. and Arfanis, M. and Falaras, P. and Kitsiou, P.V. and Pateras, I. and Bergonzini, A. and Frisan, T. and Kyriazis, S. and Tsoukleris, D.S. and Tsilibary, E.-P.C. and Gazouli, M. and Pavlatou, E.A. and Gorgoulis, V.G.",
    journal = "Cancer Genomics and Proteomics",
    year = "2021",
    volume = "18",
    pages = "425-439",
    publisher = "International Institute of Anticancer Research",
    doi = "10.21873/CGP.20269",
    keywords = "silver nanoparticle;  titanium dioxide nanoparticle;  nanomaterial;  silver;  titanium;  titanium dioxide, antineoplastic activity;  apoptosis;  Article;  biological activity;  cell culture;  cell viability;  colorimetry;  cytotoxicity;  DNA fragmentation assay;  human;  human cell;  MCF-7 cell line;  MDA-MB-231 cell line;  MTT assay;  photoactivation;  protein expression;  sol-gel;  Western blotting;  chemistry;  nanomedicine;  neoplasm;  procedures, Humans;  Nanomedicine;  Nanostructures;  Neoplasms;  Silver;  Titanium",
    abstract = "Background/Aim: Nanomedicine is a promising scientific field that exploits the unique properties of innovative nanomaterials, providing alternative solutions in diagnostics, prevention and therapeutics. Titanium dioxide nanoparticles (TiO2NPs) have a great spectrum of photocatalytic antibacterial and anticancer applications. The chemical modification of TiO2optimizes its bioactive performance. The aim of this study was the development of silver modified NPs (Ag/TiO2NPs) with anticancer potential. Materials and Methods: Ag/TiO2NPs were prepared through the sol-gel method, were fully characterized and were tested on cultured breast cancer epithelial cells (MCF-7 and MDAMB-231). The MTT colorimetric assay was used to estimate cellular viability. Western blot analysis of protein expression along with a DNA-laddering assay were employed for apoptosis detection. Results and Conclusion: We show that photo-activated Ag/TiO2NPs exhibited significant cytotoxicity on the highly malignant MDA-MB-231 cancer cells, inducing apoptosis, while MCF-7 cells that are characterized by low invasive properties were unaffected under the same conditions. © 2021 International Institute of Anticancer Research. All rights reserved."
}