@article{3077296,
    title = "Comparative e-cadherin digital expression analysis in HPV and non-HPV related squamous cell carcinoma of the oral cavity",
    author = "CHRYSOVERGIS, A. and PAPANIKOLAOU, V. and MASTRONIKOLIS, N. and SPYROPOULOU, D. and ADAMOPOULOU, M. and TSIAMBAS, E. and PESCHOS, D. and RAGOS, V. and KYRODIMOS, E.",
    journal = "ANTICANCER RESEARCH",
    year = "2021",
    volume = "41",
    number = "1",
    pages = "163-167",
    publisher = "International Institute of Anticancer Research",
    issn = "0250-1291",
    doi = "10.21873/anticanres.14761",
    keywords = "uvomorulin;  cadherin;  tumor marker, Article;  cancer patient;  cancer staging;  clinical feature;  controlled study;  data analysis software;  digital imaging;  down regulation;  female;  histopathology;  human;  human tissue;  image analysis;  immunohistochemistry;  male;  mouth squamous cell carcinoma;  papillomavirus infection;  priority journal;  protein expression level;  tissue section;  Alphapapillomavirus;  cancer grading;  complication;  disease predisposition;  gene expression;  genetics;  immunohistochemistry;  metabolism;  mouth tumor;  papillomavirus infection;  pathology;  squamous cell carcinoma;  virology, Alphapapillomavirus;  Biomarkers, Tumor;  Cadherins;  Carcinoma, Squamous Cell;  Disease Susceptibility;  Female;  Gene Expression;  Humans;  Immunohistochemistry;  Male;  Mouth Neoplasms;  Neoplasm Grading;  Neoplasm Staging;  Papillomavirus Infections",
    abstract = "Background/Aim: Oral squamous cell carcinoma (OSCC) demonstrates aggressive biological behavior in subgroups of patients with specific molecular characteristics. Concerning metastatic potential, disruption of cell to cell adhesion is a critical event in epithelial malignancies including OSCC. Our aim was to investigate the role of E-Cadherin expression in OSCC patients as a valuable protein marker. Materials and Methods: Fifty (n=50) tissue sections derived from primary OSCCs were analyzed by implementing an immunohistochemistry (IHC) assay based on a proper anti-Ecadherin antibody. Digital image analysis was also implemented for an objective evaluation of the corresponding protein expression levels. Results: E-cadherin altered expression (low to negative) was observed in 34/50 (68%) cases, whereas the rest (16/50-32%) demonstrated normal (high to moderate) expression. E-Cadherin abnormal expressionwas associated with the stage of the examined malignancies (p=0.023), whereas no significant correlations with grade, gender, smoking status or human papilloma virus (HPV) history were observed. Conclusion: E-Cadherin down regulation is frequently observed in OSCC and is correlated to a progressively aggressive phenotype of the malignancy in the corresponding patients (advanced stage), but it seems that the impact of HPV persistent infection on these patients is not a critical parameter. © 2021 International Institute of Anticancer Research. All rights reserved."
}