@article{3077855, title = "MicroRNA-92a-3p overexpression in peripheral blood mononuclear cells is an independent predictor of prolonged overall survival of patients with chronic lymphocytic leukemia", author = "Papageorgiou, S.G. and Diamantopoulos, M.A. and Kontos, C.K. and Bouchla, A. and Vasilatou, D. and Bazani, E. and Scorilas, A. and Pappa, V.", journal = "Clinical Lymphoma Myeloma and Leukemia", year = "2019", volume = "60", number = "3", pages = "658-667", publisher = "Taylor and Francis Ltd.", doi = "10.1080/10428194.2018.1461861", keywords = "lactate dehydrogenase; microRNA; microRNA 92a 3p; unclassified drug; immunoglobulin heavy chain; microRNA; MIRN92 microRNA, human; tumor marker, adult; aged; amplicon; Article; blood donor; cancer prognosis; cancer survival; chronic lymphatic leukemia; cohort analysis; female; flow cytometry; follow up; housekeeping gene; human; human cell; immunophenotyping; in vitro study; intermediate risk patient; lactate dehydrogenase blood level; major clinical study; male; mRNA expression level; overall survival; peripheral blood mononuclear cell; polyadenylation; priority journal; protein expression; real time polymerase chain reaction; reverse transcription; Sanger sequencing; signal transduction; U-937 cell line; very elderly; case control study; chronic lymphatic leukemia; gene expression regulation; genetics; Kaplan Meier method; metabolism; middle aged; mononuclear cell; mortality; mutation; prognosis; proportional hazards model; tumor cell line, Aged; Aged, 80 and over; Biomarkers, Tumor; Case-Control Studies; Cell Line, Tumor; Female; Gene Expression Regulation, Neoplastic; Humans; Immunoglobulin Heavy Chains; Kaplan-Meier Estimate; Leukemia, Lymphocytic, Chronic, B-Cell; Leukocytes, Mononuclear; Male; MicroRNAs; Middle Aged; Mutation; Prognosis; Proportional Hazards Models", abstract = "MicroRNA-92a-3p (miR-92a-3p) derives from the oncogenic miR-17/92 cluster and its highly homologous miR-106a/363 cluster. miR-92a-3p regulates the expression of key transcription factors such as HIF1 and inhibits SOCS1 to enhance the anti-apoptotic STAT3/IL6 signaling pathway. In this study, we assessed the putative usefulness of miR-92a-3p as a prognostic and/or diagnostic biomarker in chronic lymphocytic leukemia (CLL). For this purpose, total RNA was extracted from mononuclear cells isolated from the peripheral blood of 88 CLL patients and 36 non-leukemic blood donors, was polyadenylated and reversely transcribed. miR-92a-3p expression was quantified using an accurate qPCR method. miR-92a-3p levels were significantly lower in peripheral blood mononuclear cells of CLL patients. Overall survival (OS) analysis revealed that high miR-92a-3p expression predicts significantly prolonged OS of CLL patients. Interestingly, miR-92a-3p overexpression remains a significant prognosticator in subgroups of CLL patients with distinct prognosis. In conclusion, miR-92a-3p overexpression is a potential surrogate biomarker of favorable outcome of CLL patients. © 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group." }