@article{3078333,
    title = "Expression of circulating micrornas linked to bone metabolism in chronic kidney disease-mineral and bone disorder",
    author = "Yavropoulou, M.P. and Vaios, V. and Makras, P. and Georgianos, P. and Batas, A. and Tsalikakis, D. and Tzallas, A. and Ntritsos, G. and Roumeliotis, S. and Eleftheriadis, T. and Liakopoulos, V.",
    journal = "BioMedicine (France)",
    year = "2020",
    volume = "8",
    number = "12",
    pages = "1-15",
    publisher = "MDPI AG",
    doi = "10.3390/biomedicines8120601",
    abstract = "The pathophysiology of chronic kidney disease–mineral and bone disorder (CKD-MBD) is complex and multifactorial. Recent studies have identified a link between microRNAs (miRNAs) and bone loss. In this study, we investigated the expression of miRNAs in CKD-MBD. In this case-control study, we included thirty patients with CKD-MBD (cases) and 30 age-and gender-matched healthy individuals (controls). Bone mineral density (BMD) and trabecular bone score (TBS) evaluation was performed with dual X-ray absorptiometry. The selected panel of miRNAs included: hsa-miRNA-21-5p; hsa-miRNA-23a-3p; hsa-miRNA-24-2-5p; hsa-miRNA-26a-5p; hsa-miRNA-29a-3; hsa-miRNA-124-3p; hsa-miRNA-2861. The majority of cases had low BMD values. The relative expression of miRNA-21-5p was 15 times lower [fold regulation (FR): −14.7 ± 8.1, p = 0.034), miRNA-124-3p, 6 times lower (FR: −5.9 ± 4, p = 0.005), and miRNA-23a-3p, 4 times lower (FR: −3.8 ± 2.0, p = 0.036) in cases compared to controls. MiRNA-23a-3p was significantly and inversely correlated with TBS, adjusted for calcium metabolism and BMD values (beta = −0.221, p = 0.003, 95% CI −0.360, −0,081) in cases. In a receiver operating characteristic (ROC) analysis, expression of miRNA-124-3p demonstrated 78% sensitivity and 83% specificity in identifying CKD patents with osteoporosis. Serum expression of miRNAs related to osteoblasts (miRNA-23a-3p) and osteoclasts (miRNA-21-5p, miRNA-124-3p) is significantly altered in patients with CKD-MBD. © 2020 by the authors. Licensee MDPI, Basel, Switzerland. article distributed under the terms and conditions of the Cre."
}