@article{3081880,
    title = "Mutation detection of the human glucocorticoid receptor alpha gene area
coding for the hormone-binding domain by denaturing gradient gel
electrophoresis",
    author = "Tsolakidou, AF and Coulocheri, SA and Sekeris, CE and Moutsatsou, P",
    journal = "Journal of Biochemical and Biophysical Methods",
    year = "2002",
    volume = "51",
    number = "3",
    pages = "233-241",
    publisher = "ELSEVIER SCIENCE BV",
    issn = "0165-022X",
    doi = "10.1016/S0165-022X(02)00012-X",
    keywords = "DGGE; glucocorticoid receptor; mutation detection",
    abstract = "Mutations in the hormone-binding domain of the human glucocorticoid
receptor alpha (hGRalpha) gene have been detected in a variety of
glucocorticoid resistance syndromes. Using the denaturing gradient gel
electrophoresis technique, we developed a sensitive method for the
detection of alterations in the gene area coding for the whole
hormone-binding domain and part of the DNA-binding domain of the
hGRalpha. This method can be applied for screening of glucocorticoid
receptor gene alterations in glucocorticoid-dependent diseases. (C) 2002
Elsevier Science B.V. All tights reserved."
}