@article{3082614,
    title = "Adenovirus-mediated expression of antisense MMP-9 in glioma cells
inhibits tumor growth and invasion",
    author = "Lakka, SS and Rajan, M and Gondi, C and Yanamandra, N and Chandrasekar, and N and Jasti, SL and Adachi, Y and Siddique, K and Gujrati, M and and Olivero, W and Dinh, DH and Kouraklis, G and Kyritsis, AP and Rao, JS",
    journal = "Oncogenesis",
    year = "2002",
    volume = "21",
    number = "52",
    pages = "8011-8019",
    publisher = "Nature Publishing Group",
    issn = "2157-9024",
    doi = "10.1038/sj.onc.1205894",
    keywords = "ECM; MMP-9; MT-MMP; adenovirus; antisense; glioma",
    abstract = "Matrix metalloproteinase 9 (MMP-9) is known to play a major role in cell
migration and invasion in both physiological and pathological processes.
Our previous work has shown that increased MMP-9 levels are associated
with human glioma tumor progression. In this study, we evaluated the
ability of an adenovirus containing a 528 bp cDNA sequence in antisense
orientation to the 5’ end of the human MMP-9 gene (Ad-MMP-9AS) to
inhibit the invasiveness and migratory capacity of the human
glioblastoma cell line SBN19 in in vitro and in vivo models. Infection
of glioma cells with Ad-MMP-9AS reduced MMP-9 enzyme activity by
approximately 90% compared with mock- or Ad-CMV-infected cells.
Migration and invasion of glioblastoma cells infected with Ad-MMP-9AS
were significantly inhibited relative to Ad-CMV-infected controls in
spheroid and Matrigel assays. Intracranial injections of SNB19 cells
infected with Ad-MMP-9AS did not produce tumors in nude mice. However,
injecting the Ad-MMP-9AS construct into subcutaneous U87MG tumors in
nude mice caused regression of tumor growth. These results support the
theory that adenoviral-mediated delivery of the MMP-9 gene in the
antisense orientation has therapeutic potential for treating gliomas."
}