@article{3086061,
    title = "Proteomic analysis of normal and cancer cervical cell lines reveals deregulation of cytoskeleton-associated proteins",
    author = "Pappá, K.I. and Lygirou, V. and Kontostathi, G. and Zoidakis, J. and Makridakis, M. and Vougas, K. and Daskalakis, G. and Polyzos, A. and Anagnou, N.P.",
    journal = "Cancer Genomics and Proteomics",
    year = "2017",
    volume = "14",
    number = "4",
    pages = "253-266",
    publisher = "International Institute of Anticancer Research",
    doi = "10.21873/cgp.20036",
    keywords = "actin;  actin depolymerizing factor;  cell extract;  cofilin 1;  cytoskeleton protein;  cytoskeleton protein, actin filament;  Article;  bioinformatics;  carcinogenesis;  cervical cancer cell line;  controlled study;  female;  HeLa cell line;  human;  human cell;  Human papillomavirus type 16;  Human papillomavirus type 18;  in vitro study;  keratinocyte;  matrix assisted laser desorption ionization time of flight mass spectrometry;  protein analysis;  protein expression;  protein function;  proteomics;  signal transduction;  two dimensional electrophoresis;  upregulation;  uterine cervix;  virus strain;  Wart virus;  Western blotting;  matrix-assisted laser desorption-ionization mass spectrometry;  metabolism;  pathology;  tumor cell line;  two dimensional gel electrophoresis;  uterine cervix tumor, Cell Line, Tumor;  Cytoskeletal Proteins;  Electrophoresis, Gel, Two-Dimensional;  Female;  Humans;  Proteomics;  Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization;  Uterine Cervical Neoplasms",
    abstract = "Background: Both HPV-positive and -negative cervical cancers are primarily associated with features of cell cycle and cytoskeletal disruption; however, the actual biological processes affected remain elusive. To this end, we systematically characterized the intracellular proteomic profiles of four distinct and informative cervical cell lines. Materials and Methods: Cell extracts from a normal cervical (HCK1T) and three cervical cancer cell lines, one HPVnegative (C33A), and two HPV-positive, SiHa (HPV16+) and HeLa (HPV18+), were analyzed by 2-dimensional electrophoresis and differentially expressed proteins were identified by MALDI-TOF mass spectrometry, while differential expression was confirmed by western blot analysis. Results: In total, 133 proteins were found differentially expressed between the normal and the cervical cancer lines. Bioinformatics analysis revealed the actin cytoskeleton signaling pathway to be significantly affected, while up-regulation of cofilin-1, an actin depolymerizing factor, was documented and further validated by western blotting. Furthermore, two-way comparisons among the four cell lines, revealed a set of 18 informative differentially expressed proteins. Conclusion: These novel identified proteins provide the impetus for further functional studies to dissect the mechanisms operating in the two distinct pathways of cervical carcinogenesis."
}