@article{3086272,
    title = "miR-26a Mediates Adipogenesis of Amniotic Fluid Mesenchymal Stem/Stromal Cells via PTEN, Cyclin E1, and CDK6",
    author = "Trohatou, O. and Zagoura, D. and Orfanos, N.K. and Pappa, K.I. and Marinos, E. and Anagnou, N.P. and Roubelakis, M.G.",
    journal = "Stem Cells and Development",
    year = "2017",
    volume = "26",
    number = "7",
    pages = "482-494",
    issn = "1547-3287, 1557-8534",
    doi = "10.1089/scd.2016.0203",
    keywords = "CCNE1 protein, human;  CDK6 protein, human;  cyclin dependent kinase 6;  cyclin E;  microRNA;  MIRN26A microRNA, human;  oncoprotein;  phosphatidylinositol 3,4,5 trisphosphate 3 phosphatase;  PTEN protein, human, adipocyte;  adipogenesis;  adipose tissue;  amnion fluid;  bone development;  cell culture;  cytology;  genetics;  human;  mesenchymal stroma cell;  metabolism;  multipotent stem cell, Adipocytes;  Adipogenesis;  Adipose Tissue;  Amniotic Fluid;  Cells, Cultured;  Cyclin E;  Cyclin-Dependent Kinase 6;  Humans;  Mesenchymal Stromal Cells;  MicroRNAs;  Multipotent Stem Cells;  Oncogene Proteins;  Osteogenesis;  PTEN Phosphohydrolase",
    abstract = "Recent findings indicate that microRNAs (miRNAs) are critical for the regulatory network of adipogenesis in human mesenchymal stem/stromal cells (MSCs). Fetal MSCs derived from amniotic fluid (AF-MSCs) represent a population of multipotent stem cells characterized by a wide range of differentiation properties that can be applied in cell-based therapies. In this study, miRNA microarray analysis was performed to assess miRNA expression in terminal differentiated AF-MSCs into adipocyte-like cells (AL cells). MiR-26a was identified in high expression levels in AL cells indicating a critical role in the process of adipogenesis. Overexpression of miR-26a in AF-MSCs led to significant induction of their adipogenic differentiation properties that were altered after miR-26a inhibition. We have demonstrated that miR-26a regulates adipogenesis through direct inhibition of PTEN, which in turn promotes activation of Akt pathway. Also, miR-26a modulates cell cycle during adipogenesis by interacting with Cyclin E1 and CDK6. These results point to the regulatory role of miR-26a and its target genes PTEN, Cyclin E1, and CDK6 in adipogenic differentiation of AF-MSCs, providing a basis for understanding the mechanisms of fat cell development and obesity."
}