@article{3086487,
    title = "Secretory vesicle polar sorting, endosome recycling and cytoskeleton organization require the AP-1 complex in aspergillus nidulans",
    author = "Martzoukou, O. and Diallinas, G. and Amillis, S.",
    journal = "FRONTIERS IN GENETICS",
    year = "2018",
    volume = "209",
    number = "4",
    pages = "1121-1138",
    publisher = "Genetics Society of America",
    doi = "10.1534/genetics.118.301240",
    keywords = "clathrin;  septin;  transcription factor AP 1;  fungal protein;  transcription factor AP 1, Article;  Aspergillus nidulans;  cell membrane;  controlled study;  cytoplasm;  cytoskeleton;  endosome;  fungus growth;  fungus hyphae;  microtubule;  nonhuman;  priority journal;  protein localization;  secretory vesicle;  Aspergillus nidulans;  cytoskeleton;  endosome;  genetics;  growth, development and aging;  metabolism;  protein transport;  secretory vesicle, Aspergillus nidulans;  Cell Membrane;  Clathrin;  Cytoskeleton;  Endosomes;  Fungal Proteins;  Microtubules;  Protein Transport;  Secretory Vesicles;  Septins;  Transcription Factor AP-1",
    abstract = "The AP-1 complex is essential for membrane protein traffic via its role in the pinching-off and sorting of secretory vesicles (SVs) from the trans-Golgi and/or endosomes. While its essentiality is undisputed in metazoa, its role in simpler eukaryotes seems less clear. Here, we dissect the role of AP-1 in the filamentous fungus Aspergillus nidulans and show that it is absolutely essential for growth due to its role in clathrin-dependent maintenance of polar traffic of specific membrane cargoes toward the apex of growing hyphae. We provide evidence that AP-1 is involved in both anterograde sorting of RabERab11-labeled SVs and RabA/BRab5-dependent endosome recycling. Additionally, AP-1 is shown to be critical for microtubule and septin organization, further rationalizing its essentiality in cells that face the challenge of cytoskeleton-dependent polarized cargo traffic. This work also opens a novel issue on how nonpolar cargoes, such as transporters, are sorted to the eukaryotic plasma membrane. © 2018 by the Genetics Society of America."
}