@article{3086576,
    title = "Effect of polyphenols from coffee and grape on gene expression in myoblasts",
    author = "Priftis, A. and Goutzourelas, N. and Halabalaki, M. and Ntasi, G. and Stagos, D. and Amoutzias, G.D. and Skaltsounis, L.A. and Kouretas, D.",
    journal = "Mechanisms of Ageing and Development",
    year = "2018",
    volume = "172",
    pages = "115-122",
    publisher = "Elsevier Ireland Ltd",
    issn = "0047-6374",
    doi = "10.1016/j.mad.2017.11.015",
    keywords = "3 chlorogenic acid;  5 chlorogenic acid;  caftaric acid;  catechin;  cyanidin;  cyanidin 3 glucoside;  delphinidin;  epicatechin;  gallic acid;  malvidin chloride;  myrtillin;  oenin;  peonidin;  petunidin;  polyphenol;  quercetin;  resveratrol;  unclassified drug;  muscle protein;  polyphenol, 8430408g22rik gene;  acta2 gene;  actg2 gene;  aldh3a1 gene;  animal cell;  aqp5 gene;  Article;  ccdc134 gene;  ccl7 gene;  ccl9 gene;  ccnb1 ge4ne;  ccnb1 gene;  chemical composition;  coffee;  concentration (parameters);  controlled study;  crlf1 gene;  csda gene;  cyp1b1 gene;  DNA microarray;  esd gene;  fam180a gene;  fez1 gene;  gene;  gene expression;  gpr176 gene;  grape;  gsta1 gene;  gsta2 gene;  gsta4 gene;  gstm1 gene;  lgals3bp gene;  liquid chromatography-mass spectrometry;  loc235860 gene;  loc329575 gene;  mass spectrometry;  mgp gene;  mgst2 gene;  mouse;  myl9 gene;  myoblast;  nefm gene;  nnmt gene;  nonhuman;  nqo1 gene;  nrep gene;  ogn gene;  oxidation reduction state;  plf gene;  plf2 gene;  plf3 gene;  plscr2 gene;  prf1 gene;  priority journal;  psmd12 gene;  psme2b gene;  rnu6 gene;  sapcd2 gene;  slc40a1 gene;  spon2 gene;  thbs1 gene;  tmem45a gene;  txn1 gene;  ugt1a10 gene;  ugt1a9 gene;  ultra performance liquid chromatography;  biosynthesis;  cell line;  chemistry;  drug effect;  gene expression regulation;  human;  metabolism;  myoblast;  Vitis, Cell Line;  Coffee;  Gene Expression Regulation;  Humans;  Muscle Proteins;  Myoblasts;  Polyphenols;  Vitis",
    abstract = "Coffee and grape contain various bioactive compounds like polyphenols that may exert beneficial effects, especially antioxidant activity, on human health upon consumption. However, the molecular mechanisms through which these effects are achieved are not fully elucidated. Thus, in the present study in order to investigate these mechanisms, a whole genome expression DNA microarray analysis was carried out in myoblasts treated with polyphenols of coffee and grape pomace at concentrations that improved the redox status. Grape was composed of catechin, epicatechin, cyanidin, malvidin, delphinidin, petunidin, myrtillin, kuromanin, oenin, peonidin, quercetin, gallic acid and caftaric acid as LC–MS revealed, with a total polyphenolic content (TPC) of 648 mg of gallic acid equivalents/g of dry matter. Coffee had a TPC of 42.61 mg GAE/g coffee and was composed of 3-chlorogenic acid (16.61 mg/g), 4- and 5-chlorogenic acids (13.62 mg/g), as UHPLC-HRMS revealed. According to the results, grape polyphenols altered mainly the expression of cytoskeleton and differentiation-associated genes, while coffee compounds had a more profound effect, on the expression levels of many metabolic and antioxidant genes possibly through the nuclear factor (erythroid-derived 2) like-2 (Nrf2) pathway. © 2017 Elsevier B.V."
}