@article{3086882,
    title = "Oxidative stress in ageing and disease development studied by FT-IR spectroscopy",
    author = "Anastassopoulou, J. and Kyriakidou, M. and Kyriazis, S. and Mavrogenis, A.F. and Mamareli, V. and Mamarelis, I. and Petra, M. and Malesiou, E. and Kotoulas, C. and Kolovou, P. and Koutoulakis, E. and Markouizou, A. and Theophanides, T.",
    journal = "Mechanisms of Ageing and Development",
    year = "2018",
    volume = "172",
    pages = "107-114",
    publisher = "Elsevier Ireland Ltd",
    issn = "0047-6374",
    doi = "10.1016/j.mad.2017.11.009",
    keywords = "carbonyl derivative;  peroxide, adult;  aortic valve;  Article;  beta sheet;  bone biopsy;  bone cancer;  bone malformation;  child;  collagen fibril;  controlled study;  disease course;  disease exacerbation;  ex vivo study;  Fourier transform infrared spectroscopy;  human;  human tissue;  irradiation;  lipid degradation;  lipid peroxidation;  lipophilicity;  oxidative stress;  priority journal;  protein conformation;  radiation dose;  aging;  aortic valve stenosis;  bone tumor;  calcinosis;  female;  femoral head;  heterotopic ossification;  infrared spectroscopy;  male;  metabolism;  pathology;  preschool child, Adult;  Aging;  Aortic Valve;  Aortic Valve Stenosis;  Bone Neoplasms;  Calcinosis;  Child;  Child, Preschool;  Female;  Femur Head;  Humans;  Male;  Ossification, Heterotopic;  Oxidative Stress;  Spectroscopy, Fourier Transform Infrared",
    abstract = "FT-IR spectroscopy was used to investigate the effect of oxidative stress and to approach the mechanism on cancer bone demineralization, aortic valve mineralization and heterotopic ossification on disease development. The FT-IR spectra obtained from paediatric, adult bone and ex vivo irradiated adult healthy bone with a dose of 20 Gy were compared with those of healthy bone. The increase of band intensity changes of vasCH2 vsCH2 in the region 3000–2850 cm−1 depended on aging, the disease progression and the dose of irradiation. The bands at 3080 cm−1 and 1744 cm−1, which originate from olefinic terminal bond (v = CH) and ester carbonyl group (vRO–C[dbnd]O), respectively, indicate the influence of oxidative stress on lipid degradation and peroxidation, respectively. The new bands at about 1690 cm−1 and 1516 cm−1 denote the presence of β-sheet conformation of the proteins due to the diseases, confirming the increasing amount of lipophilic environment and fibril formation. Comparison of the FT-IR spectra of calcified aortic valve and hip heterotopic ossification with that of normal bones showed that in the bone-like formation the peroxide anion free radicals play an important role in the disease. © 2017 Elsevier B.V."
}