@article{3087724,
    title = "HLA class II antigen expression in colorectal carcinoma tumors as a favorable prognostic marker",
    author = "Sconocchia, G. and Eppenberger-Castori, S. and Zlobec, I. and Karamitopoulou, E. and Arriga, R. and Coppola, A. and Caratelli, S. and Spagnoli, G.C. and Lauro, D. and Lugli, A. and Han, J. and Iezzi, G. and Ferrone, C. and Ferlosio, A. and Tornillo, L. and Droeser, R. and Rossi, P. and Attanasio, A. and Ferrone, S. and Terracciano, L.",
    journal = "Neoplasia (United States)",
    year = "2014",
    volume = "16",
    number = "1",
    pages = "31-42",
    publisher = "W B SAUNDERS CO-ELSEVIER INC",
    doi = "10.1593/neo.131568",
    keywords = "biological marker;  gamma interferon;  HLA antigen class 2;  interleukin 1beta;  interleukin 6, antigen expression;  article;  cancer cell culture;  cancer prognosis;  clinical feature;  colorectal carcinoma;  controlled study;  disease course;  enzyme linked immunosorbent assay;  flow cytometry;  gene expression;  human;  human cell;  immunohistochemistry;  immunoperoxidase staining;  in vitro study;  pathology;  peripheral blood mononuclear cell;  priority journal;  retrospective study;  reverse transcription polymerase chain reaction;  RNA extraction;  tissue microarray, Carcinoma;  Cell Line, Tumor;  Colorectal Neoplasms;  Disease Progression;  Female;  Gene Expression Profiling;  Gene Expression Regulation, Neoplastic;  Histocompatibility Antigens Class II;  HLA-DP Antigens;  HLA-DQ Antigens;  HLA-DR Antigens;  Humans;  Inflammation;  Interferon-gamma;  Interleukin-1beta;  Interleukin-6;  Intestinal Mucosa;  Male;  Monocytes;  Neoplasm Metastasis;  Oligonucleotide Array Sequence Analysis;  Prognosis",
    abstract = "The goal of this study was to determine the frequency of HLA class II antigen expression in colorectal carcinoma (CRC) tumors, its association with the clinical course of the disease, and the underlying mechanism(s). Two tissue microarrays constructed with 220 and 778 CRC tumors were stained with HLA-DR, DQ, and DP antigen-specific monoclonal antibody LGII-612.14, using the immunoperoxidase staining technique. The immunohistochemical staining results were correlated with the clinical course of the disease. The functional role of HLA class II antigens expressed on CRC cells was analyzed by investigating their in vitro interactions with immune cells. HLA class II antigens were expressed in about 25% of the 220 and 21% of the 778 tumors analyzed with an overall frequency of 23%. HLA class II antigens were detected in 19% of colorectal adenomas. Importantly, the percentage of class II antigen staining was weakly detected only in 5.4% of 37 normal mucosa tissues. HLA class II antigen expression was associated with a favorable clinical course of the disease. In vitro stimulation with interferon gamma (IFNγ) induced HLA class II antigen expression on two of the four CRC cell lines tested. HLA class II antigen expression on CRC cells triggered interleukin-1β (IL-1β) production by resting monocytes. HLA class II antigen expression in CRC tumors is a favorable prognostic marker. This association may reflect stimulation of IL-1β production by monocytes. © 2014 Neoplasia Press, Inc. All rights reserved."
}