@article{3088927,
    title = "14-3-3 gene expression exerts isoform-dependent functions in sinonasal pathophysiology",
    author = "Kataki, A. and Alevizos, L. and Lazaris, A. and Glustianou, G. and Derventzi, A. and Giotakis, E. and Konstadoulakis, M. and Yiotakis, I.",
    journal = "PATHOLOGY RESEARCH AND PRACTICE",
    year = "2014",
    volume = "210",
    number = "2",
    pages = "105-110",
    publisher = "Elsevier GmbH",
    issn = "0344-0338",
    doi = "10.1016/j.prp.2013.10.006",
    keywords = "cycline;  protein 14 3 3;  stratifin;  isoprotein;  protein 14 3 3;  YWHAB protein, human, 14 3 3 beta gene;  14 3 3 gene;  14 3 3 sigma gene;  adult;  aged;  apoptosis;  article;  basal cell;  cell proliferation;  controlled study;  cytoplasm;  female;  flow cytometry;  gene;  gene expression;  human;  human tissue;  immunohistochemistry;  inverted papilloma;  major clinical study;  male;  nose polyp;  paranasal sinus disease;  respiratory epithelium;  upregulation;  adolescent;  cohort analysis;  epithelium cell;  gene expression regulation;  metabolism;  middle aged;  nose mucosa;  nose tumor;  papilloma;  pathophysiology;  very elderly;  young adult, 14-3-3 Proteins;  Adolescent;  Adult;  Aged;  Aged, 80 and over;  Apoptosis;  Cell Proliferation;  Cohort Studies;  Epithelial Cells;  Female;  Gene Expression Regulation, Neoplastic;  Humans;  Male;  Middle Aged;  Nasal Mucosa;  Nasal Polyps;  Nose Neoplasms;  Papilloma;  Papilloma, Inverted;  Protein Isoforms;  Young Adult",
    abstract = "The expression profiles of 14-3-3β and θ isoforms, known to exert both oncogenic and antiapoptotic effects, were assessed in different entities of nasal pathophysiology.Flow cytometry and immunohistochemistry were used on paraffin-embedded sections of 51 inverted papillomas (IP), 26 nasal polyps (NP), 9 polyps with IP (NPIP) and 10 specimens of normal epithelium (NE).14-3-3β expression was significantly upregulated in IP as compared with both NP ( p= 0.015) and NE ( p= 0.002). 14-3-3β was also increased in NPIP as compared with NE ( p= 0.008). 14-3-3β cytoplasmic staining was more pronounced in basal cells of the respiratory epithelium although serous glands and the vascular system were often positive as well. High 14-3-3β immunopositivity in IP patients concurred with increased proliferative activity shown by PCNA immunostaining ( p= 0.04). Expression of 14-3-3θ was also found increased in IP and NPIP patients, compared to NP ( p= 0.005, p= 0.002 respectively) and NE ( p= 0.004 and p= 0.001 respectively). 14-3-3θ cytoplasmic immunopositivity was detected in columnar epithelium, particularly in basal and subluminal cells, whereas no immunoreactivity was observed in NP and NE.Our results demonstrate differential expression of 14-3-3β and θ isoforms in sinonasal pathophysiology, supporting their implication, respectively, in the proliferative and inflammatory process engaged in the formation of IP. © 2013 Elsevier GmbH."
}