@article{3089195,
    title = "Ursolic acid triggers apoptosis and Bcl-2 downregulation in MCF-7 breast cancer cells",
    author = "Kassi, E. and Sourlingas, T.G. and Spiliotaki, M. and Papoutsi, Z. and Pratsinis, H. and Aligiannis, N. and Moutsatsou, P.",
    journal = "Cancer Investigation",
    year = "2009",
    volume = "27",
    number = "7",
    pages = "723-733",
    issn = "0735-7907, 1532-4192",
    doi = "10.1080/07357900802672712",
    keywords = "caspase 3;  caspase 7;  caspase activated deoxyribonuclease;  dexamethasone;  DFF45 protein;  glucocorticoid receptor;  luciferase;  mifepristone;  nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase;  protein bcl 2;  transcription factor AP 1;  triamcinolone;  unclassified drug;  ursolic acid, apoptosis;  article;  breast cancer;  cell nucleus;  cell strain MCF 7;  cell viability;  controlled study;  drug cytotoxicity;  drug isolation;  flow cytometry;  gel mobility shift assay;  gene expression;  genetic transfection;  HeLa cell;  human;  human cell;  mitochondrion;  priority journal;  protein cleavage;  protein DNA binding;  Salvia officinalis;  transcription regulation;  Western blotting, Active Transport, Cell Nucleus;  Adenocarcinoma;  Antineoplastic Agents;  Apoptosis;  Binding, Competitive;  Breast Neoplasms;  Cell Line, Tumor;  Dexamethasone;  Down-Regulation;  Drug Screening Assays, Antitumor;  Female;  Gene Expression Regulation, Neoplastic;  Genes, bcl-2;  Hela Cells;  Humans;  Mifepristone;  Neoplasm Proteins;  Poly(ADP-ribose) Polymerases;  Proto-Oncogene Proteins c-bcl-2;  Receptors, Glucocorticoid;  Transcription Factor AP-1;  Transcription, Genetic;  Triamcinolone;  Triterpenes",
    abstract = "In this report we determine the ability of ursolic acid (UA) to induce apoptosis and to modulate glucocorticoid receptor (GR) and Activator Protein-1 (AP-1) in MCF-7 cells. The UA-induced apoptosis (53 μM), the PARP cleavage, and the decrease in Bcl-2 protein (53 μM) support the notion that UA induces apoptosis through the intrinsic mitochondrial pathway. UA binds GR (relative binding affinity: 2.57) and translocates GR into nucleus, suggesting its potential as a GR modulator. UA had no effect on GRE- or TRE-driven gene expression. In summary, UA is a GR modulator and may be considered as a potential anticancer agent in breast cancer. Copyright © Informa Healthcare USA, Inc."
}