@article{3090487,
    title = "A modified chromatin-immunoprecipitation protocol for silkmoth ovarian follicular cells reveals C/EBP and GATA binding modes on an early chorion gene promoter",
    author = "Papantonis, A. and Lecanidou, R.",
    journal = "Current Molecular Biology Reports",
    year = "2009",
    volume = "36",
    number = "4",
    pages = "733-736",
    issn = "2198-6428",
    doi = "10.1007/s11033-008-9236-1",
    keywords = "Animals;  Bombyx;  CCAAT-Enhancer-Binding Proteins;  Chorion;  Chromatin Immunoprecipitation;  Female;  GATA Transcription Factors;  Ovarian Follicle;  Promoter Regions, Genetic;  Time Factors, Bombyx",
    abstract = "Standard Chromatin immunoprecipitation protocols have been designed to suit studies performed on cell line cultures or yeast cells growing in liquid cultures. In these cases cross-linking/fixation takes place directly in the growing medium of the cells by the addition of a general fixation reagent. When applied on whole isolated silkmoth follicles, this procedure results in poor release of follicular cells from the basal membrane and lower yield of cross-linked chromatin. We present a modification to the standard protocol, where detachment of follicular cells from the basal membrane of the egg and nuclei isolation precedes formaldehyde-mediated cross-linking. We also discuss application of the modified method for the identification of distinct BmC/EBP and BmGATAβ binding modes on a chorion gene promoter from the Er1.A/B early gene pair. © 2008 Springer Science+Business Media B.V."
}