@article{3095501,
    title = "Expression of Eph receptor tyrosine kinases and their ligands in human Granulosa lutein cells and human umbilical vein endothelial cells",
    author = "Xu, Y. and Zagoura, D. and Keck, C. and Pietrowski, D.",
    journal = "Experimental and Clinical Endocrinology and Diabetes",
    year = "2006",
    volume = "114",
    number = "10",
    pages = "590-595",
    doi = "10.1055/s-2006-950499",
    keywords = "chorionic gonadotropin;  ephrin A4;  ephrin B1;  ephrin B2;  ephrin receptor A2;  ephrin receptor A4;  ephrin receptor A7;  ligand;  messenger RNA;  protein tyrosine kinase, angiogenesis;  article;  cancer cell culture;  cell stimulation;  controlled study;  corpus luteum;  endothelium cell;  female;  granulosa cell;  HeLa cell;  human;  human cell;  luteal cell;  priority journal;  protein expression;  reverse transcription polymerase chain reaction;  umbilical vein, Cell Separation;  DNA Primers;  Endothelium, Vascular;  Female;  Gene Expression Regulation, Enzymologic;  Granulosa Cells;  Humans;  Pregnancy;  Receptor, EphA1;  Reverse Transcriptase Polymerase Chain Reaction;  Umbilical Veins",
    abstract = "Corpus luteum development is regulated by gonadotropins and accompanied by extremely rapid vascularization of the avascular granulosa cell compartiment by endothelial cells (EC). The proliferation of Granulosa cells (GC) and EC is a complex interplay and takes place in a spatially and temporarily coordinated manner. The erythropoietin-producing hepatoma amplified sequence (Eph) receptors and their ligands-the ephrins- are a recently detected family of membrane located protein tyrosine kinases which play a crucial role in the growth and development of nerve and blood vessel network. We report about the mRNA expression pattern of Ephs and their ligands in human GC, in human EC, and in carcinoma cell lines OvCar-3 and Hela. The mRNA of EphA4, EphA7, ephrinA4, ephrinB1 and ephrinB2 was detected in GC and EC, while EphA2 was expressed only in CC. The expression of various Ephs and ephrins did not change in GC after stimulation with human chorion gonadotropin. Our study analyzes for the first time the expression of the complete human Eph/ephriny-system in GC and in EC. The remarkable similarity between these two cell types supports the theory of a functional relationship of EC and GC. In addition, it was shown that hCG is not a major determinant of Eph/ephrin regulation in GC. © J. A. Barth Verlag in Georg Thieme Verlag KG."
}