@article{3095715,
    title = "Potential role of apoptosis and apoptotic regulatory proteins in colorectal neoplasia: Correlations with clinico-pathological parameters and survival",
    author = "Tzouvala, M. and Lazaris, A.C. and Papatheodoridis, G.V. and Kouvidou, C. and Papathomas, T.G. and Kavantzas, N. and Elemenoglou, I. and Karamanolis, D.G. and Agapitos, E.",
    journal = "Digestive Diseases and Sciences",
    year = "2008",
    volume = "53",
    number = "2",
    pages = "451-460",
    issn = "0163-2116, 1573-2568",
    doi = "10.1007/s10620-007-9857-6",
    keywords = "protein bcl 2;  protein MDM2;  protein p53, adult;  aged;  apoptosis;  article;  cancer survival;  colorectal cancer;  controlled study;  correlation analysis;  dysplasia;  female;  histopathology;  human;  human tissue;  immunohistochemistry;  immunophenotyping;  major clinical study;  male;  nick end labeling;  prediction;  priority journal;  prognosis;  proportional hazards model;  protein expression;  villous adenoma, Adenoma;  Adult;  Aged;  Apoptosis;  Colorectal Neoplasms;  Female;  Humans;  Image Processing, Computer-Assisted;  Immunohistochemistry;  Immunophenotyping;  In Situ Nick-End Labeling;  Male;  Middle Aged;  Prognosis;  Proportional Hazards Models;  Proto-Oncogene Proteins c-bcl-2;  Proto-Oncogene Proteins c-mdm2;  Tumor Suppressor Protein p53",
    abstract = "An imbalance between apoptotic and proliferative processes is believed to underlie colorectal neoplasia. We evaluated the expression of bcl-2, p53, mdm2 proteins, and apoptosis in colorectal neoplasms, as well as their correlation with clinico-pathological parameters, using image analysis. Biopsies from 46 colorectal cancers, 121 adenomas, and 25 controls were studied using monoclonal antibodies against p53, bcl-2, mdm2 and the terminal deoxynucleotidyl- transferase-mediated dUTP nick end labeling (TUNEL) method for apoptosis. P53 and bcl2 protein expression was higher in adenomas ≥1 cm (P < 0.03) and tubulovillous-villous adenomas (P < 0.03), and correlated with dysplasia (P < 0.03). In Cox regression analysis, Dukes' stage was the most significant independent prognostic indicator of a worse survival (P < 0.019), whereas when stage was eliminated, bcl-2 expression was also a powerful predictor for bad prognosis (P = 0.02). In conclusion, both bcl-2 and p53 immunohistochemical profiles may be useful adjuncts in detecting adenomas with a malignant potential, whereas bcl-2 could be used in combination with Dukes' stage as a predictor of prognosis in colorectal cancer. © 2007 Springer Science+Business Media, LLC."
}