@article{3101314,
    title = "Absence of human rhinovirus and respiratory syncytial virus from bronchoalveolar lavage and bronchial biopsies of selected patients with stable chronic obstructive pulmonary disease",
    author = "Giannakaki, S. and Politi, L. and Antonogiannaki, E.M. and Spanakis, N. and Arsenis, G. and Filaditaki, V. and Zakynthinos, S. and Karagiannidis, N. and Tsakris, A.",
    journal = "BMJ Open Respiratory Research",
    year = "2016",
    volume = "17",
    number = "1",
    publisher = "BioMed Central Ltd.",
    issn = "2052-4439",
    doi = "10.1186/s12931-016-0323-x",
    keywords = "virus RNA, adult;  age distribution;  aged;  anthropometry;  arterial gas;  blood carbon dioxide tension;  Body mass airflow Obstruction Dyspnea Exercise scale;  bronchus biopsy;  chronic obstructive lung disease;  clinical article;  comorbidity;  computer assisted tomography;  controlled study;  coronary artery disease;  coughing;  diabetes mellitus;  disease severity;  dyspnea;  Editorial;  female;  fiberoptic bronchoscopy;  forced vital capacity;  Greece;  hemoptysis;  human;  Human respiratory syncytial virus;  human tissue;  hyperlipidemia;  hypertension;  lung adenocarcinoma;  lung cancer;  lung function;  lung lavage;  male;  modified Medical Research Council scale;  population research;  rating scale;  real time polymerase chain reaction;  Rhinovirus;  small cell lung cancer;  smoking;  smoking habit;  spirometry;  squamous cell lung carcinoma;  stable chronic obstructive lung disease;  symptomatology;  university hospital;  bronchoalveolar lavage fluid;  bronchus;  Human respiratory syncytial virus;  isolation and purification;  middle aged;  pathology;  Pulmonary Disease, Chronic Obstructive;  Rhinovirus;  severity of illness index;  sputum;  virology, Aged;  Bronchi;  Bronchoalveolar Lavage Fluid;  Female;  Humans;  Male;  Middle Aged;  Pulmonary Disease, Chronic Obstructive;  Respiratory Syncytial Viruses;  Rhinovirus;  Severity of Illness Index;  Sputum",
    abstract = "Previous studies have reported very different rates of human rhinovirus (HRV) and respiratory syncytial virus (RSV) genome detection in nasal and sputum samples, but not in bronchoalveolar lavage (BAL) and bronchial biopsy samples. Our study aimed to investigate the presence of HRV and RSV in the lungs of 31 consecutive patients with stable COPD (11 GOLD stage I, 11 II, and 9 III) and 22 control subjects (12 current or past smokers, and 10 non-smokers), who underwent diagnostic (e.g., lung cancer) and/or therapeutic (e.g., hemoptysis) fibreoptic bronchoscopy in a university hospital in Athens, Greece. Viral RNA of HRV and RSV were not detected in any of the samples of COPD patients or control subjects after being processed with real-time PCR. © 2016 Giannakaki et al."
}