@article{3116400,
    title = "The role of liquid-based cytology in the investigation of colorectal
lesions: a cytohistopathological correlation and evaluation of
diagnostic accuracy",
    author = "Kontzoglou, Konstantinos and Moulakakis, Konstantinos G. and Alexiou, and Dimitrios and Safioleas, Michael C. and Nikiteas, Nikolaos and Giahnaki, and Anna-Eva and Kyroudes, Aspasia and Kostakis, Alkiviadis and Karakitsos, and Petros",
    journal = "Langenbeck's Archives of Surgery",
    year = "2007",
    volume = "392",
    number = "2",
    pages = "189-195",
    publisher = "Springer-Verlag",
    issn = "1435-2443, 1435-2451",
    doi = "10.1007/s00423-006-0120-y",
    keywords = "colonic brushing; brush cytology; biopsy; thin-prep; liquid-based",
    abstract = "Background amd aims: The role of cytologic techniques is not widely
accepted even if it is well documented that the cytologic investigation
of colorectal tract could complement the biopsy and increase the correct
diagnosis of carcinomas. This study aims to evaluate the role of
Thin-Prep (R) liquid-based cytology in the investigation of colorectal
lesions.
Materials and methods: We compared the diagnostic accuracy of Thin-Prep
(R) with that of biopsy in 158 patients with signs and symptoms of the
lower gastrointestinal tract. Each patient underwent colonoscopy,
followed by tissue biopsy and brush cytology. Brushing material was
obtained and prepared according to the operating manual of Thin-Prep
(R).
Results: The rate of unsatisfactory smears was 5% (8/158) with
liquid-based technique and appeared to be slightly higher than the
respective of biopsy [3.2% (5/158) (5 vs 3.2%, P = 0.18)]. Based on
the final diagnosis, 89 out of 93 total malignant cases and 53 out of 53
total benign cases were correctly diagnosed with Thin-Prep (R) technique
(four false negatives). Accurate diagnosis with biopsy was performed in
87 out of 93 total malignant cases and 53 out of 53 total benign cases
with biopsy (six false negatives). Neither in Thin-Prep (R) technique
nor in biopsy were false-positive cases observed. The sensitivities of
detecting malignancy by Thin-Prep (R) and biopsy were 95.7, and 93.5%,
respectively (no significant difference, P = 0.239). The sensitivity was
augmented (98.9%) when the two techniques were combined, and this
difference was found to be statistically significant (98.9 vs 92.9, P =
0.01, and 98.9 vs 95.7, P = 0.039).
Conclusions: Liquid-based cytology appears to be an easy, highly
accurate, and reliable cytologic method for the diagnostic approach of
colorectal diseases and could be applied as complementary to biopsy for
the improvement of the diagnosis. Moreover, could be used for DNA ploidy
analysis and immmunohistochemical studies."
}