@article{3126234,
    title = "Associations of combined polymorphisms of the platelet membrane glycoproteins Ia and IIIa and the platelet-endothelial cell adhesion molecule-1 and P-Selectin genes with IVF implantation failures",
    author = "Vlachadis, N. and Tsamadias, V. and Vrachnis, N. and Kaparos, G. and Vitoratos, N. and Kouskouni, E. and Economou, E.",
    journal = "JOURNAL OF OBSTETRICS AND GYNAECOLOGY",
    year = "2017",
    volume = "37",
    number = "3",
    pages = "363-369",
    publisher = "Taylor and Francis Ltd.",
    issn = "0144-3615",
    doi = "10.1080/01443615.2016.1256978",
    keywords = "alpha2 integrin;  CD31 antigen;  glycoprotein IIIa;  PADGEM protein;  alpha2 integrin;  beta3 integrin;  biological marker;  CD31 antigen;  PADGEM protein, adult;  allele;  Article;  controlled study;  embryo transfer;  female;  gene locus;  genetic association;  genetic heterogeneity;  genetic risk;  genotype;  human;  major clinical study;  nidation;  nulligravida;  priority journal;  protein polymorphism;  pyrosequencing;  secondary analysis;  age;  area under the curve;  blood;  case control study;  genetics;  heterozygote;  in vitro fertilization;  nidation;  risk;  sensitivity and specificity;  single nucleotide polymorphism;  treatment failure, Adult;  Age Factors;  Antigens, CD31;  Area Under Curve;  Biomarkers;  Case-Control Studies;  Embryo Implantation;  Female;  Fertilization in Vitro;  Heterozygote;  Humans;  Integrin alpha2;  Integrin beta3;  P-Selectin;  Polymorphism, Single Nucleotide;  Risk;  Sensitivity and Specificity;  Treatment Failure",
    abstract = "The aim of the study was to investigate the combined impact of the genetic heterogeneity of the glycoproteins Ia (GpIa) and IIIa (GpIIIa) and the platelet-endothelial cell adhesion molecule-1 (PECAM-1) and P-Selectin genes on IVF embryo transfer implantation failures (IVF-ET failures). Sixty nulligravida women with previous IVF-ET failures and 60 fertile controls were genotyped for the GpIa-C807T, GpIIIa-PlA1/PA2, PECAM-1-C373G (Leu125Val) and P-Selectin-A37674C (Thr715Pro) polymorphisms by pyrosequencing. Compared with wild-type combined homozygotes, carriers of combinations of risk alleles in two gene loci were at significantly increased risk for IVF-ET failure, whereas carriers of the combination of GpIa-807T, GpIIIa-PlA2 and PECAM-1-373G alleles had OR = 52.50 (95%CI: 4.05–680.95, p <.001). The area under the receiver-operating characteristic curve (AUC) based on the number of polymorphisms and the number of risk alleles per subject was 75.4% (95%CI: 66.7%–82.8%, p <.001) and 72.5% (95%CI: 63.6%–80.3%, p <.001), respectively. The OR per polymorphism and risk allele increase was 4.26 (95%CI: 2.15–8.41, p <.001) and 2.85 (95%CI: 1.71–4.76, p <.001), respectively. The above associations were more robust among younger women. The combined analysis of these polymorphisms revealed strong association of combined carriers with IVF-ET failures especially for younger women and provided a genetic risk score with good diagnostic accuracy in the prediction of IVF-ET failures. © 2017 Informa UK Limited, trading as Taylor & Francis Group."
}