@article{3130312,
    title = "Temporal and spatial expression of TGF-β1 in an Achilles tendon section model after application of platelet-rich plasma",
    author = "Lyras, D.N. and Kazakos, K. and Tryfonidis, M. and Agrogiannis, G. and Botaitis, S. and Kokka, A. and Drosos, G. and Tilkeridis, K. and Verettas, D.",
    journal = "Foot and Ankle Surgery",
    year = "2010",
    volume = "16",
    number = "3",
    pages = "137-141",
    publisher = "Elsevier Ireland Ltd",
    issn = "1268-7731, 1460-9584",
    doi = "10.1016/j.fas.2009.09.002",
    keywords = "transforming growth factor beta1;  transforming growth factor beta1, achilles tendon;  animal cell;  animal experiment;  animal model;  animal tissue;  article;  cell structure;  controlled study;  down regulation;  fibroblast;  histopathology;  image analysis;  immunohistochemistry;  nonhuman;  priority journal;  protein analysis;  protein expression;  rabbit;  surgical approach;  surgical technique;  thrombocyte rich plasma;  upregulation;  wound healing;  achilles tendon;  animal;  Ankle Injuries;  biosynthesis;  comparative study;  disease model;  metabolism;  pathology;  physiology;  rupture, Achilles Tendon;  Animals;  Ankle Injuries;  Disease Models, Animal;  Immunohistochemistry;  Platelet-Rich Plasma;  Rabbits;  Rupture;  Transforming Growth Factor beta1;  Wound Healing",
    abstract = "Background: To investigate the effect of platelet-rich plasma (PRP) on TGF-β1 expression during tendon healing. Methods: We used 48 skeletally mature New Zealand White rabbits. 24 rabbits received the PRP, and 24 rabbits served as an untreated control group. Equal numbers of animals were sacrificed at 1st, 2nd, 3rd, and 4th week. The surgical procedure involved a transverse incision to transect the Achilles tendon. A volume of 1. ml of PRP was then injected into the tendon mass in the PRP group. Histological and immunohistochemical evaluations with an anti-TGF-β primary antibody were performed. Results: The pattern of expression of TGF-β1 in the PRP group was characterized by a significant upregulation during the first 2 weeks and subsequently significant downregulation in the 3rd and 4th week in comparison with the controls. Conclusions: Our results suggest that PRP may affect the tendon healing process by altering the expression of TGF-β1. © 2009 European Foot and Ankle Society."
}