@article{3132316,
    title = "Liver histology alterations during carbon dioxide pneumoperitoneum in a porcine model",
    author = "Alexakis, N. and Gakiopoulou, H. and Dimitriou, C. and Albanopoulos, K. and Fingerhut, A. and Skalistira, M. and Patsouris, E. and Bramis, J. and Leandros, E.",
    journal = "SURGICAL ENDOSCOPY AND OTHER INTERVENTIONAL TECHNIQUES",
    year = "2008",
    volume = "22",
    number = "2",
    pages = "415-420",
    doi = "10.1007/s00464-007-9440-4",
    keywords = "carbon dioxide, abdominal pressure;  animal experiment;  animal model;  animal tissue;  aorta flow;  artery blood flow;  article;  control group;  controlled study;  edema;  female;  heart rate;  hepatic artery;  hyperemia;  inflammation;  liver biopsy;  liver histology;  male;  mean arterial pressure;  nonhuman;  pneumoperitoneum;  portal vein blood flow;  priority journal;  statistical significance;  swine;  tissue injury, Animals;  Carbon Dioxide;  Female;  Liver Diseases;  Male;  Models, Animal;  Pneumoperitoneum, Artificial;  Swine",
    abstract = "Background: This study aimed to investigate the time course changes in liver histology during carbon dioxide (CO2) pneumoperitoneum in a large animal model. Methods: For this study, 14 white pigs were anesthetized. Liver biopsies performed 0, 1, and 2 h after establishment of CO2 pneumoperitoneum (at 12 mmHg) and after peritoneal desufflation were sent for histologic examination. Heart rate, mean blood pressure, hepatic artery flow, portal vein flow, and aortic flow were recorded in 10-min increments. Three animals served as control subjects. Results: A statistically significant time course increase was observed in portal inflammation, intralobular inflammation, edema, sinusoidal dilation, sinusoidal hyperemia, centrilobular dilation, centrilobular hyperemia, pericentrilobular ischemia, and focal lytic necrosis scores. There were no significant changes in the control group. This eliminated an effect of anesthesia only. The portal vein flow increased as much as 21%, and the hepatic artery flow decreased as much as 31% of baseline, but these differences did not attain statistical significance. Aortic flow remained relatively stable. Conclusion: Histomorphologic changes occurred, indicating liver tissue injury during CO2 pneumoperitoneum at an intraabdominal pressure of 12 mmHg in the porcine model. Portal vein flow increased, and hepatic artery flow decreased, whereas aortic flow remained relatively unaffected in this experiment. © 2007 Springer Science+Business Media, LLC."
}