@article{3146405,
    title = "Acute Hyperhomocysteinemia Impairs Endothelium Function in Subjects with
Type 2 Diabetes Mellitus",
    author = "Doupis, J. and Eleftheriadou, I. and Kokkinos, A. and Perrea, D. and and Pavlatos, S. and Gonis, A. and Katsilambros, N. and Tentolouris, N.",
    journal = "Experimental and Clinical Endocrinology & Diabetes",
    year = "2010",
    volume = "118",
    number = "7",
    pages = "453-458",
    publisher = "JOHANN AMBROSIUS BARTH VERLAG MEDIZINVERLAGE HEIDELBERG GMBH",
    issn = "0947-7349, 1439-3646",
    doi = "10.1055/s-0030-1248290",
    keywords = "homocysteine; type 2 diabetes mellitus; flow-mediated vasodilation;
augmentation index; arterial stiffness",
    abstract = "The objective of the present study was to examine the effect of acute,
methionine-induced hyper-homocysteinemia (HHCY) on endothelial function
and indices of arterial stiffness in subjects with type 2 diabetes
mellitus (T2DM). A total of 30 subjects with T2DM, free of macrovascular
disease were examined in a crossover study. L-methionine (M) (0.1 g/kg)
and water (W) load were given in random order with an interval of about
1 week in between. Endothelial function was assessed by flow-mediated
vasodilation (FMD). Arterial stiffness was assessed by determination of
augmentation index (AI). Measurements were performed in the fasting
state, 1, 2 and 3 h after the M or the W load. Total plasma homocysteine
(HCY) levels did not change after W administration, while M
administration resulted in a significant increase in HCY concentrations
at 3 h. FMD throughout the experiment expressed as area under the curve
(AUC) was significantly lower after the M than after the W load.
Consistent with impairment in endothelial function, the AUC of AI was
significantly higher after the M than after the W administration. Acute
HHCY impairs endothelial function and increases arterial stiffness in
patients with T2DM. This effect is probably mediated by a reduction of
nitric oxide bioavailability in endothelium."
}