@article{3168729, title = "West Nile Virus Circulation in Mosquitoes in Greece (2010-2013)", author = "Patsoula, Eleni and Vakali, Annita and Balatsos, Georgios and and Pervanidou, Danai and Beleri, Stavroula and Tegos, Nikolaos and Baka, and Agoritsa and Spanakos, Gregory and Georgakopoulou, Theano and Tserkezou, and Persefoni and Van Bortel, Wim and Zeller, Herve and Menounos, Panagiotis and and Kremastinou, Jenny and Hadjichristodoulou, Christos", journal = "BioMed Research International", year = "2016", volume = "2016", publisher = "HINDAWI LTD", issn = "2314-6133, 2314-6141", doi = "10.1155/2016/2450682", abstract = "Background of the Study. Following a large West Nile virus (WNV) epidemic in Northern Greece in 2010, an active mosquito surveillance system was implemented, for a 3-year period (2011, 2012, and 2013). Description of the Study Site and Methodology. Using mainly CO2 mosquito traps, mosquito collections were performed. Samples were pooled by date of collection, location, and species and examined for the presence of WNV. Results. Positive pools were detected in different areas of the country. In 2010, MIR and MLE values of 1.92 (95% CI: 0.00-4.57) and 2.30 (95% CI: 0.38-7.49) were calculated for the Serres Regional Unit in Central Macedonia Region. In 2011, the highest MIR value of 3.71(95% CI: 1.52-5.91) was recorded in the Regions of Central Greece and Thessaly. In 2012, MIR and MLE values for the whole country were 2.03 (95% CI: 1.73-2.33) and 2.15 (95% CI: 1.86-2.48), respectively, for Cx. pipiens. In 2013, in the Regional Unit of Attica, the one outbreak epicenter, MIR and MLE values for Cx. pipiens were 10.75 (95% CI: 7.52-13.99) and 15.76 (95% CI: 11.66-20.65), respectively. Significance of Results/Conclusions. The contribution of a mosquito-based surveillance system targeting WNV transmission is highlighted through the obtained data, as in most regions positive mosquito pools were detected prior to the date of symptom onset of human cases. Dissemination of the results on time to Public Health Authorities resulted in planning and application of public health interventions in local level." }