@article{3169068,
    title = "Characterisation of IncA/C-2 plasmids carrying an In416-like integron
with the bla(VIM-19) gene from Klebsiella pneumoniae ST383 of Greek
origin",
    author = "Papagiannitsis, Costas C. and Dolejska, Monika and Izdebski, Radoslaw and and Giakkoupi, Panagiota and Skalova, Anna and Chudejova, Katerina and and Dobiasova, Hana and Vatopoulos, Alkiviadis C. and Derde, Lennie P. G. and and Bonten, Marc J. M. and Gniadkowski, Marek and Hrabak, Jaroslav",
    journal = "International Journal of Antimicrobial Agents",
    year = "2016",
    volume = "47",
    number = "2",
    pages = "158-162",
    publisher = "ELSEVIER SCIENCE BV",
    issn = "0924-8579",
    doi = "10.1016/j.ijantimicag.2015.12.001",
    keywords = "IncA/C-2 plasmids; CMY; VIM; Class 1 integron",
    abstract = "The complete nucleotide sequences of three multidrug resistance (MDR)
IncA/C-like plasmids from Enterobacteriaceae isolates carrying the
VIM-type carbapenemase-encoding integrons In4863
(bla(VIM-19)-aacA7-dfrA1-Delta aadA1-smr2) or In4873
(bla(VIM-1)-aacA7-dfrA1-Delta aadA1-smr2) were determined, which are the
first In416-like elements identified in Greece. Plasmids pKP-Gr642 and
pKP-Gr8143 were from Klebsiella pneumoniae ST383 isolates, whereas
plasmid pEcl-Gr4873 was from an Enterobacter cloacae ST88 isolate.
Sequencing showed that pKP-Gr642 (162 787 bp) and pKP-Gr8143 (154 395
bp) consisted of the type 1 IncA/C-2 conserved backbone, the
blaCMY-2-like gene-containing region, and the ARI-B (with the sul2 gene)
and ARI-A (with a class 1 integron) resistance islands, like the plasmid
pUMNK88-161 from the USA. The third plasmid, pEcl-Gr4873 (153 958 bp),
exhibited extensive similarity with the type 2 IncA/C-2 plasmid pR55
from France. pEcl-Gr4873 carried only one resistance island of a hybrid
transposon structure inserted in a different location to ARI-A in type 1
A/C-2 plasmids. In all three plasmids, the In416-like integrons In4863
or In4873 were identified within non-identical class II transposon
structures. All three In416-like-carrying regions presented significant
similarities with the MDR region of the IncA/C-2 plasmid pCC416 from
Italy, carrying the prototype In416 integron
(bla(VIM-4)-aacA7-dfrA1-Delta aadA1-smr2). These findings provided the
basis for speculations regarding the evolution of IncA/C-2 plasmids with
In416-like integrons, and confirmed the rapid evolution of some IncA/C-2
plasmid lineages. Considering the broad host range of IncA/C-2
molecules, it seems that pKP-Gr642, pKP-Gr8143 and pEcl-Gr4873 plasmids
might support the diffusion of In416-like integrons among
Enterobacteriaceae. (C) 2015 Elsevier B.V. and the International Society
of Chemotherapy. All rights reserved."
}